摘要
Objective: The aims of this research were to purify and identify the 1 30 kDa (CagA) protein of H. pylori clinical isolate HP97002 and evaluate the rel ationships between the purified 130 kDa (CagA) protein and gastric diseases. Met hods: The procedure for isolating the protein included 6 mol/L guanidine extract , size exclusion chromatography and elusion from gel. Sera of 68 patients with g astric diseases (44 with chronic gastritis,15 with atrophic gastritis,7 with p eptic ulcer disease,2 with gastric cancer ) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Result s : The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gast ritis 86.7% (13/15),peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ 2=13.327, P =0.004), and 130 kDa (CagA) protein was associated with severe gastric disease s (r_s=0.442, \%P\%=0.001). Conclusion: The 130 kDa (CagA) protein was asso ciated with severe gas tric diseases.
Objective:The aims of this research were to purify and identify the 130 kDa(CagA) protein of H.pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa(CagA) pro-tein and gastric diseases.Methods:The procedure for isolating the protein included 6 mol/L guanidine ex-tract,size exclusion chromatography and elusion from gel.Sera of 68 patients with gastric diseases(44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer) were obtained,and the serological response to CagA was studied by Westem-blot using the purified protein,Results;The pu-rified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera,43 sera could recognize the purified protein associated with chronic agastritis 47.7%(21/44),atrophic gastritis 86.7%(13/15),peptic ulcer disease 100%(7/7),gastric cancer 100%(2/2).Compared with each other,the difference was significant(x^2=13.327,P=0.004),and 130 kDa(CagA) protein was associated with severe gastric diseases(rs=0.442,P=0.001).Conclusion:The 130 kDa(CagA) protein was associated wih severe gastric diseases.
