生物荧光氧化硅纳米颗粒的研制与应用

Synthesis and Applications of Florescence-Doped Silica Nanoparticles

运用OP-10(壬基酚聚氧乙烯醚)/环己烷/氨水微乳液自组装体系合成了罗丹明B嵌入的荧光氧化硅纳米颗粒.通过电镜检测、光谱分析以及荧光猝灭试验研究了荧光颗粒的特性.将荧光纳米颗粒与培养细胞共培育后,通过激光扫描共聚焦显微镜和流式细胞仪研究了不同时间点细胞内荧光信号和荧光强度.结果表明,合成的荧光氧化硅纳米颗粒粒径小(约20nm),分布均匀,形态规则,表面光滑圆润,发光性质稳定.它可被体外培养细胞有效摄入,并可在培养细胞中检测到较强的荧光信号和较高的荧光强度.这提示生物荧光氧化硅纳米颗粒在细胞生物学、超微化学与免疫检测等领域将具有重要应用前景.

The fluorescence-doped Silica nanoparticles (FDS-NP) were synthesized in a microemulsion system of polyoxyethylene nonylphenyl ether (OP-10)/ cyclohexane/ammonium hydroxide. To elucidate the character of FDS-NP, techniques of electron microscopy, analysis of spectrum and collisional quenching of fluorescence were used. In order to study the fluorescent signal and intensity of FDS-NP in the cultured cells, laser-scanning confocal microscope and the analysis of flow cytometry were utilized. The results showed that the size of FDS-NP was very small (about 20 nm), the distribution was narrow, the shape was circular and lubricity, and the fluorescence was photostable. Cultured cells could efficiently uptake FDS-NP, and yield high fluorescent signal and intensity. All these indicated that FDS-NP is an important nanoparticulate biomarker, and is applicable to many areas of modem biochemical analysis, such as ultrasensitive detection, immunoassys, as well as cellular biology.