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肝癌患者外周血AFP mRNA和TSGF的检测及意义 被引量:3

CLINICAL SIGNIFICANCE OF ALPHA FETOPROTEIN mRNA IN PERIPHERAL BLOOD AND TUMOR SPECIFIC GROWTH FACTORS IN SERA OF PATIENTS WITH PRIMARY HEPATOCELLULER CARCINOMA
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摘要 目的 :检测原发性肝细胞癌 (PHC)患者外周血单个核细胞表达 AFP m RNA情况和血清肿瘤特异性生长因子 (TSGF)的含量及探讨其临床意义。方法 :采用逆转录聚合酶链反应 (RT- PCR)检测 86例 PHC患者外周血 AFP m RNA,同时采用 TS-GF试剂盒检测患者血清 TSGF含量。结果 :PHC患者外周血 AFP m RNA阳性率为 5 3 .5 % (46/86) ,血清 TSGF阳性率为80 .2 % (69/86) ,AFP m RNA阳性率与 TSGF含量呈显著正相关 (r =0 .6415 ,P <0 .0 5 )。外周血 AFP m RNA阳性率、血清TSGF含量及阳性率均与肿瘤分期、癌灶大小及肝癌肝外转移显著正相关 (均为 P <0 .0 5 )。结论 :PHC患者外周血 AFP m R-NA阳性率与血清 TSGF水平呈显著正相关 ,两者均与肿瘤分期、癌灶大小及癌转移呈显著正相关。检测外周血 AFP m RNA和血清 Objective:To detect the expression of alphal fetoprotein mRNA(AFP mRNA) in peripheral blood and the level of tumor specific growth factors (TSGF) in sera of patients with primary hepatocelluler carcinoma (PHC), and analyse their clinical significance.Methods:AFP mRNA was amplified by nested RT PCR and the level of TSGF was measured by TSGF diagnostic reagent kit.Results:The positive rate of AFP mRNA and of TSGF was 53.5% and 80.2%, respectively. The positive rate of AFP mRNA had a remarkable correlation with the level of TSGF in serum, and they both were correlated strongly with the size and metastases of tumor (P<0.05). The positive rate of AFP mRNA (76.5%) and the average level of TSGF (88.7±22.4) U/ml in serum in present metastasis group were significant higher than those of absent metastasis group [38.5% and (72.1±13.8) U/ml,P<0.01 and P<0.01,respectively].Conclusion:There is a significant correlation between the positive rate of AFP mRNA and the level of TSGF in PHC patients' sera, and they are correlated strongly with tumor metastases. AFP mRNA in peripheral blood and the level of TSGF in serum are likely to be clinically useful in evaluating the risk of developing in or extrahepatic metastases of HCC tumor cells.
出处 《广西医科大学学报》 CAS 2003年第1期10-12,共3页 Journal of Guangxi Medical University
基金 广西区自然科学基金资助项目 (9732 0 2 9)
关键词 肝癌 患者 外周血 AFPMRNA TSGF 检测 肿瘤特异性生长因于 liver cancer alphal fetoprotein mRNA tumor specific growth facters (TSGF)
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  • 1李桂生,杨春旭,李平.肺癌标志物在临床实际工作中的作用[J].国外医学(肿瘤学分册),1996,23(5):298-301. 被引量:10
  • 2丁远锵 唐琳 等.恶性肿瘤特异性生长因子检测的临床应用[J].福建医学检验,1997,2(3):106-106.
  • 3汪欣,福建医学检验,1997年,2卷,1期,10页
  • 4徐元斌,福建医学检验,1996年,1卷,3期,118页
  • 5柏华,血清肿瘤标志物对肺癌的诊断价值,1995年,1卷,1期,23页
  • 6汤钊猷,现代肿瘤学,1993年,87页
  • 7赵洪涛.TSGF临床检测初探[J].福建医药杂志.1999(06)
  • 8华一兵,陈涛,马利民,蒋理.胃肠道恶性肿瘤TSGF测定及其价值的初步评估[J].福建医药杂志.1999(06)
  • 9高贞国.TSGF在健康人群筛查中的价值[J].福建医药杂志.1999(06)
  • 10朱根海,杨舒盈,陈春英.评多项肿瘤标记物对卵巢癌的诊断价值[J].中国现代医学杂志,1999,9(11):7-8. 被引量:7

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