摘要
目的 :检测原发性肝细胞癌 (PHC)患者外周血单个核细胞表达 AFP m RNA情况和血清肿瘤特异性生长因子 (TSGF)的含量及探讨其临床意义。方法 :采用逆转录聚合酶链反应 (RT- PCR)检测 86例 PHC患者外周血 AFP m RNA,同时采用 TS-GF试剂盒检测患者血清 TSGF含量。结果 :PHC患者外周血 AFP m RNA阳性率为 5 3 .5 % (46/86) ,血清 TSGF阳性率为80 .2 % (69/86) ,AFP m RNA阳性率与 TSGF含量呈显著正相关 (r =0 .6415 ,P <0 .0 5 )。外周血 AFP m RNA阳性率、血清TSGF含量及阳性率均与肿瘤分期、癌灶大小及肝癌肝外转移显著正相关 (均为 P <0 .0 5 )。结论 :PHC患者外周血 AFP m R-NA阳性率与血清 TSGF水平呈显著正相关 ,两者均与肿瘤分期、癌灶大小及癌转移呈显著正相关。检测外周血 AFP m RNA和血清
Objective:To detect the expression of alphal fetoprotein mRNA(AFP mRNA) in peripheral blood and the level of tumor specific growth factors (TSGF) in sera of patients with primary hepatocelluler carcinoma (PHC), and analyse their clinical significance.Methods:AFP mRNA was amplified by nested RT PCR and the level of TSGF was measured by TSGF diagnostic reagent kit.Results:The positive rate of AFP mRNA and of TSGF was 53.5% and 80.2%, respectively. The positive rate of AFP mRNA had a remarkable correlation with the level of TSGF in serum, and they both were correlated strongly with the size and metastases of tumor (P<0.05). The positive rate of AFP mRNA (76.5%) and the average level of TSGF (88.7±22.4) U/ml in serum in present metastasis group were significant higher than those of absent metastasis group [38.5% and (72.1±13.8) U/ml,P<0.01 and P<0.01,respectively].Conclusion:There is a significant correlation between the positive rate of AFP mRNA and the level of TSGF in PHC patients' sera, and they are correlated strongly with tumor metastases. AFP mRNA in peripheral blood and the level of TSGF in serum are likely to be clinically useful in evaluating the risk of developing in or extrahepatic metastases of HCC tumor cells.
出处
《广西医科大学学报》
CAS
2003年第1期10-12,共3页
Journal of Guangxi Medical University
基金
广西区自然科学基金资助项目 (9732 0 2 9)