缺氧诱导乳鼠心肌细胞凋亡的观察

OBSERVATION OF APOPTOSIS OF CULTURED NEONATAL RAT CARDIOMYOCYTES INDUCED BY HYPOXIA

目的 :观察缺氧对乳鼠心肌细胞凋亡的诱导作用。方法 :体外培养的乳鼠心肌细胞 ,分组选择不同的缺氧时间 ,应用流式细胞仪 (FCM)检测心肌细胞凋亡比例。结果 :1PI染色 FCM检测 ,缺氧 13 .5、14、15、16、16.5 h亚二倍体细胞比例分别为1.8%、3 .5 %、6.8%、3 .4 %、1.4 % (P <0 .0 5～ 0 .0 1) ;2 Annexin V- FITC/ PI双染色 FCM检测 ,缺氧 12、12 .5、13、13 .5、14、14 .5 h早期凋亡细胞比例分别为 10 .7%、13 .7%、14 .5 %、15 %、16.7%、17.2 % (P <0 .0 1) ,其余缺氧时间和正常对照组细胞未见明显细胞凋亡。结论

Objective:To observe the effect of apoptosis of cardiomyocytes induced by hypoxia Methods:Neonatal rat cardiomyocytes were cultured under different time hypoxia in groups Flow cytometry(FCM) was used to detect apoptosis of cardiomyocytes Results:Analysis of hypodiploid cell using DNA FCM showed that hypodiploid cells under hypoxia condition were 1 8%,3 5%,6 8%,3 4%,1 4%(P<0 05~0 01) at 13 5,14,15,16,16 5 hours, respectively The percentage of early phase apoptotic cells stained with Annexin V FITC/PI was 10 7%,13 7%,14 5%,15%,16 7%,17 2%(P<0 01) at 12,12 5,13,13 5,14,14 5 hour, respectively Other Hypoxia times and the normal control groups did not show obvious apoptotic cells Conclusion:Apoptosis of cardiomyocytes could induce by hypoxia