鸡球虫广东清远分离株对七种药物的抗药性试验

STUDY ON DRUG-RESISTANCE TO SEVEN ANTICOCCIDIA DRUGS OF CHICKEN COCCIDIAN FROM QINGYUAN, GUANGDONG

为了了解广东清远某鸡场球虫抗药性的产生程度 ,并制定出合理的用药方案 ,给延长药物的使用周期提供科学的依据 ,我们从该鸡场现场采集了新鲜粪便 ,实验室分离和繁殖卵囊 ,并在实验室进行球虫的抗药性研究。经检测粪中含堆型艾美耳球虫 (E.acervulina)和变位艾美耳球虫 (E.mivati)共 81.3 % ,柔嫩艾美耳球虫 (E.tenella) 18.7%。试验选择目前常用的 7种抗球虫药物 ,分别为球佳 (地克珠利 Diclazuril)、杜球 (马杜拉霉素 Madu-ramicin)、欲可胖 (莫能菌素 Monensin)、球安 (拉沙里霉素 Lasalocid)、氨丙啉 (Amprolian)、球克 (海南霉素 Hainan-mycin)、氯苯胍 (Robenidine)。试验设 7个药物组和感染不用药、不感染不用药 2个对照组 ,每组 10只鸡。在 15日龄 ,除不感染不用药组外 ,每组接种孢子化卵囊 12× 10 4个。以抗球虫指数 (ACI)、病变记分减少率 (RLS)、相对卵囊产量 (ROP)、最适抗球虫活性百分率 (POAA)为试验指标 ,进行综合评定。结果显示鸡球虫广东清远株对球佳为轻度抗药 ,对杜球、欲可胖、球安、氨丙啉为完全抗药 ,对球克。

We collected the excrement from the broiler farm in Qingyuan, Guangdong and isolated, propagated the coccicial oocysts in the test in order to know drug resistant degree of coccidia and make a proper program for scientific pharmaceuticals. Among the isolated occysts, the total percent of E.acervulina and E.mivati was 81.3% , E.tenella was 18.7%. We selected seven drugs to study the drug resistance of chicken coccidia in the test. The drugs included Diclazuril, Maduramicin, Monensin, Lasalocid, Amprolian, Hainanmycin and Robenidine. The experiment contained 7 medicated groups, 1 inoculated unmedicated and 1 uninoculated unmedicated controls group. There were 10 birds in every group. On fifteen day old, all groups were inoculated with 12×10 4 sporulated oocysts per bird. The criteria used to measure the sensitivity of Eimeria species included anticoccidial index(ACI), reduction of lesion score(RLS), relative oocysts production(ROP) and percent of optimum anticoccidial activity(POAA). There results indicated the isolated strain was light resistance to Diclazuril, partly resistance to Hainanmycin、 Robenidine, and complete resistance to Maduramicin、 Monensin、 Lasalocid、 Amprolian.