摘要
以农杆菌LBA4 4 0 4介导将外源基因导入玉米骨干自交系齐 319、掖 5 15、掖 5 0 2等胚性愈伤组织 ,并由筛选出的抗性愈伤组织再生出可育植株。农杆菌浓度、共培养时间和玉米的基因型显著影响农杆菌介导的玉米愈伤组织的转化率。农杆菌的浓度OD60 0 0 5～ 0 7,共培养时间 3～ 4天时转化率较高。在相同条件下不同基因型的玉米胚性愈伤组织的转化率为 3 3%～ 10 %。
Embryogenic calli of elite maize inbred Qi319, Ye515 and Ye502 were transformed via Agrobacterium tumefaciens , and plantlets were regenerated from resistant calli The concentration of Agrobacterium, duration of co-culture and the genotype of maize could affect the transformation efficiency of calli via Agrobacterium With the concentration of Agrobacterium at OD 600 0 5-0 7, and 3-4 days of co-culture, the transformation efficiency of calli was higher compared with other conditions Under identical conditions, the transformation efficiency of different genotypes of calli was 3 3%-10%
出处
《山东农业科学》
2003年第2期3-6,共4页
Shandong Agricultural Sciences
基金
国家高技术发展计划 ( 2 0 0 2AA2 12 0 71)资助
