远缘杂交后代材料的高分子量麦谷蛋白亚基组成分析

Composition of High Molecular Weight Glutenin Subunits in 89 Lines from a Distant Hybridization

以一个远缘杂交后代材料的89个株系为试材,用SDS-PAGE电泳方法鉴定分析了这些株系的高分子量麦谷蛋白亚基(HMW-GS)组成。结果表明,在后代材料中亚基组成类型极其丰富。在Glu-A1、Glu-B1和Glu-D1三个位点上分别检测到3,6和9种不同的亚基类型,3位点结合共出现30种组成类型。其中,在Glu-A1位点上,1亚基出现频率最高,为57.31%;在Glu-B1位点上7+8和7+9亚基出现频率较高,分别为44.94%和34.83%;Glu-D1位点上的变异类型最丰富,其中被世界公认的优质亚基5+10出现频率最高,达38.20%,另外,还存在一些其它新的亚基组合类型。由此看来利用远缘杂交改良我国小麦品质有一定的优势。

The compositions of highmolecularweight glutenin subunits (HMWGS) in 89 lines from a distant hybridization were analyzed using SDSPAGE method in this experiment. The results showed that the HMWGS composition of 89 lines were extremely abundant. 3,6 and 9 different HMWGS types were found in the loci of GluA1, GluB1 and GluD1, respectively. After combining of three loci, there were 30 compositions types. At GluA1 locus, the frequency of subunit 1 (57.31%) was the highest. At GluB1 locus, the frequencies of subunits 7+8 (44.94%) and subunits 7+9 (38.83%) were relatively high. Variations of HMWGS at GluD1 were the most abundant, among which the frequency of subunits 5+10 (38.20%),which were regarded as the highest quality subunits,was the highest. And there were other new allelic genes. Using distant hybridization to improve wheat quality was very useful in China.