刺激人γδT细胞增殖的结核杆菌多肽抗原的生物学特性分析

Characterization of Mycobacterium tuberculosis polypeptide antigen capable of stimulating the proliferation of human γδT cells

目的 :分析可刺激人γδT细胞增殖的结核杆菌多肽抗原(Mtb Ag)的某些生物学特性。方法 :以结核杆菌分泌蛋白与经透析或链霉蛋白酶处理的Mtb Ag体外分别刺激人外周血单个核细胞 (PBMC)增殖 ,培养 10d时 ,采用流式细胞仪分析细胞的表型。结果 :透析后的Mtb Ag刺激γδT细胞增殖的活性未见明显降低 ;而经链霉蛋白酶处理的Mtb Ag促进γδT细胞增殖的活性则显著下降。另外 ,与结核杆菌分泌蛋白相比较 ,Mtb Ag刺激γδT细胞增殖的活性显著增强。 结论 :Mtb Ag中可刺激人γδT细胞增殖的成分是一种相对分子质量 (Mr)大于 10 0 0 0、非分泌性。

AIM: To analyse biological properties of Mycobacterium tuberculosis polypeptide antigen(Mtb Ag) that could stimulate the proliferation of human γδT cells. METHODS: Mtb secretory proteins and Mtb Ag treated via dialysis or with pronase were used to stimulate human PBMCs. After being cultured for 10 days, the phenotype of responsive cells was analysed by flow cytometry. RESULTS: The proliferation stimulating activity to human γδT cells of dialysed Mtb Ag did not decrease remarkably, whereas that of the pronase treated Mtb Ag reduced significantly. Moreover, the proliferative stimulating activity to γδT cells of Mtb Ag was notably higher than Mtb secretory protein. CONCLUSION: The component in Mtb Ag that can stimulate the proliferation of human γδT cells is a non secretory and protase sensitive polypeptides with M r over 10 000.