郑氏植物蛋白对乳腺癌及其癌前病变患者PBL的刺激作用及其意义

The effects of Zheng’s plant-protein on the proliferation of lymphocytes from patients with breast cancer and precancer

目的 :研究郑氏植物蛋白 (Zheng’splant protein ,ZPP)对淋巴细胞的刺激作用及其在乳腺癌早期诊断与治疗中的应用价值。方法 :采用3 H TdR掺入法测定人外周血淋巴细胞(PBL) )的增殖反应。用流式细胞仪测定ZPP对T淋巴细胞CD3+ 、CD4 + 和CD8+ 抗原表达的调节作用。结果 :ZPP对乳腺癌及其前期病变患者PBL有较强的刺激作用 (SI分别为3.0 0± 1.4 5和 2 .5 3± 0 .80 ) ,明显高于对正常对照组和乳管上皮轻度增生患者PBL的刺激指数 (SI分别为 1.0 6± 0 .17和1.18± 0 .19) (P <0 .0 1)。而PHA对各组PBL的增殖反应均有不同程度的刺激作用 ,但各组间无明显差异 (P >0 .0 5 ) .经ZPP刺激后的PBL中CD3+ 、CD4 + 和CD8+ 细胞均发生增殖反应 ,其中CD4 + 和CD8+ 者更为明显。结论 :ZPP可选择性刺激乳腺癌及其前期病变患者的PBL中T细胞发生增殖反应 。

AIM: To study the effect of Zheng’s plant protein(ZPP) and phytohemagglutinin(PHA) on proliferation of peripheral blood lymphocytes (PBL) and it’s application value on the early diagnosis and therapy of breast cancer. METHODS: The proliferation assay was used 3H TdR incorporation test . The phenotypes of PBL were analysised by flow cytometry(FCM). RESULTS: The ZPP significantly and select enhanced proliferation activity of PBL of primary breast and precancer cases. Their stimulation indexes(SI) were 3.00±1.45 and 2.53±0.80, while the SI of simple hyperplasia cases and normal subjecties were 1.18±0.47 and 1.06±0.91. The former were significantly higher than the latter ( P <0.01). The ZPP was also enhanced the expression level of CD3 +、CD4 + and CD8 + of PBL. CONCLUSION: This results suggest that ZPP might be a beast cancer common antigen. These activity might be used in clinical implication to diagnosis and treatment of breast cancer.