抗人跨膜激活剂及钙调亲环素配体相互作用分子单克隆抗体的制备

Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor

目的 :制备抗人跨膜激活剂及钙调亲环素配体相互作用分子(TACI)单克隆抗体 (mAb) ,研究TACI分子的细胞分布。方法 :应用淋巴细胞杂交技术制备鼠源性抗TACImAb。用免疫荧光染色、流式细胞术及免疫组织化学染色法 ,对TACI在外周血单个核细胞 (PBMC)上的分布进行鉴定。结果 :获得两株分泌抗TACImAb的杂交瘤细胞株。mAb的腹水效价达10 -7,均为IgG1亚类 ,可识别T细胞表面天然的TACI分子 ,在PHA活化的模型中 ,TACI主要表达于活化的CD4 + T细胞上。结论 :获得两株能特异性识别天然TACI分子的mAb 。

AIM: To prepare monoclonal antibodies against human TACI and study the distribution of TACI on peripheral blood nuclear cells(PBMCs). METHODS: Murine mAbs were prepared by hybridoma technique. The distribution of TACI on PBMCs were identified by immunofluorescence staining, FCM analysis as well as immunohistochemical staining. RESULTS: Two hybridoma cell lines secreting mAbs against TACI were obtained. The titers of ascitic mAbs was 10 -7 and their Ig subclasses were IgG1. Both could recognize natural TACI on T cells. In the PHA activated models, TACI was expressed mainly on activated CD4 + T cells. CONCLUSION: Two mAbs can recognize natural TACI, which will be of greater value to us in our research on the structure and functions of TACI.