芦荟提取物对衰老模型鼠抗自由基氧化损伤DNA作用的实验研究

Experimental Research of Action of Aloe Extracts on Anti-free Radical Oxidation Damage DNA in Senile Model Rats

目的 :探讨芦荟延缓衰老的疗效及其可能机理。方法 :利用小鼠吸入臭氧 (O3)制造衰老模型 ,并在其造模过程中以不同浓度的芦荟提取液实施治疗 ,9周后检测小鼠全血超氧化物歧化酶 (SOD)的活性 ,肝组织过氧化脂质代谢产物丙二醛 (MDA)的含量 ,并用彗星实验 (CometAssay)检测肝、脾组织细胞DNA氧化损伤的程度。结果 :与模型组相比 ,经芦荟治疗后 ,模型小鼠不仅全血SOD活性及肝组织MDA含量两项指标明显改善并趋向正常 ,而且肝、脾细胞DNA拖尾尾长减短 ,有效地减轻了肝、脾细胞DNA的损伤程度 ,作用较复方丹参片对照组全面。结论 :芦荟能提高机体抗自由基氧化损伤的能力 ,减轻肝、脾细胞DNA损伤程度 。

Objective: To explore the efficacy and its possible mechanism of aloe retarding senility. Methods: Senile model was made by which mice inhale ozone, treating measures of extracted liquid of aloe in different concentration was taken to administer those model mice in the course of model-making. The activity of blood SOD and the amount of MDA in liver tissue were tested and Comet Assay was applied to measure the degree of oxidation damage of cell DNA of liver and spleen tissue 9 weeks later. Results: The blood SOD activity and the amount of MDA in liver tissue were markedly higher and the length of liver and spleen cell DNA tailing was shortened in the group of mice treated by aloe extracts compared with control group. Aloe extracts efficaciously relieve the degree of liver and spleen cell DNA damage and its action is more comprehensive than Compositus Danshen tablets in the control group. Conclusion: Aloe can retard senility by which increases the capability of body anti-oxidation of free radical and relieves the damage degree of liver and spleen cell DNA.