苜蓿抗性愈伤组织诱导的初步研究

A Preliminary Study of Callus for Inducing Cold Tolerance in Alfalfa Breeding

以硫酸二乙酯 (DES)和紫外线 (U V)对准格尔苜蓿愈伤组织进行诱变处理 ,筛选其半致死浓度和剂量。对诱变处理后的材料进行脯氨酸含量测定。结果表明 ,脯氨酸含量明显高于对照。筛选出具有抗性的苜蓿愈伤组织 ,为苜蓿抗性育种提供基础材料。

MS was used as a fundamental medium for alfalfa breeding. DES and UV were successively used for callus mutation and selection. Eventually, some callus showed certain degree of cold tolerance.