米非司酮对早孕大鼠蜕膜、黄体分泌组织型纤溶酶原激活因子及蜕膜组织学的影响

Effects of Mifepristone on the Production of tPA in Cultured Rat Decidua and Corpus Luteum of Early Pregnancy and on the Morphology of Decidua

目的 进一步探讨米非司酮抗生育的作用机理。方法 应用酶联免疫吸附法检测早孕大鼠蜕膜、黄体组织培养液中组织型纤溶酶原激活因子 (tissue- type plasminogen activator,t PA)的含量 ,观察米非司酮对体外培养大鼠蜕膜形态学的影响。结果 加入米非司酮培养 2 4 h后 ,蜕膜和黄体组织培养液中的 t PA含量与对照组比较均显著升高 (P<0 .0 5 ) ;米非司酮对早孕大鼠蜕膜形态学的影响表现为蜕膜组织退化 ,间质水肿 ,血管扩张及组织间血细胞渗出。结论 提示米非司酮还可能通过激活黄体和蜕膜的纤溶过程 ,引起黄体溶解及蜕膜细胞外基质退化、蜕膜剥脱 。

Objective To further investigate the mechanism of contragestational action of mifepristone. Methods Tissue type plasminogen activator (tPA) in cultured rat decidua and corpus luteum of early pregnancy was determined by enzyme linked immunosorbent assay, and the morphology of cultured decidua was observed under a light microscope. Results The concentrations of tPA in the medium of decidua and corpus luteum were increased significantly ( P <0 05), and decidua degeneration, interstitial edema, vascular dilatation and exudation of blood cells were observed after treatment with mifepristone for 24 h. Conclusion The antigestational function of mifepristone might also be mediated by activating fibrinolysis of decidua and corpus luteum, which leads to the luteolysis and the degeneration of extra cellular matrix and shedding of rat early decidua.