摘要
目的 探讨倒卵叶五加总皂甙 (SAOH)对大鼠缺血心肌再灌注后 Bcl- 2和 Bax蛋白表达的影响。方法 采用结扎左冠状动脉前降支 30 m in,再灌注复制大鼠心肌缺血再灌注损伤 (IRI)的模型 ,结扎前 10 min注射 SAOH5 0m g/ kg、 10 0 mg/ kg,测定心肌再灌注 6 h血浆乳酸脱氢酶 (L DH)、磷酸肌酸激酶 (CK)和一氧化氮 (NO)、内皮素(ET)的含量 ,并用免疫组化的方法观察左室心肌 Bcl- 2和 Bax蛋白的表达。结果 给予 SAOH5 0 m g/ kg、 10 0 mg/ kg组较 IRI组 ,能明显减少心肌酶的漏出 (P<0 .0 1) ,显著提高血浆 NO含量 (P<0 .0 1) ,减少 ET的生成 (P<0 .0 1) ,血浆 NO与 ET比值也明显升高 (P<0 .0 1) ;SAOH亦能减少心肌 Bax蛋白、增加心肌 Bcl- 2蛋白的表达 ,Bax和 Bcl-2的比值也随之下降。结论 SAOH有明显保护心肌的作用 ,其机制之一可能与其提高血浆 NO含量、减少 ET的生成 。
Objective To study the effects of Saponin of Acanthopanax Obovatus Hoo (SAOH) on the expression of Bcl 2 and Bax proteins following myocardial ischemia reperfusion injury (IRI) in rats. Methods Ischemia reperfusion model was induced by left anterior descending coronary artery (LAD) ligation for 30 min, followed by 6 h of reperfusion. 10 min before LAD ligation, SAOH (50 mg/kg or 100 mg/kg) was administered by the route of sublingual vein injection. The level of lactate dehydrogenase (LDH), creatine kinase (CK) in myocardium, and the contents of nitric oxide (NO), endothelin (ET) in plasma were measured and the changes in the expression of Bcl 2 and Bax proteins in left ventricular myocardium after reperfusion were observed by immunohistochemical method. Results SAOH could markedly decrease the enzyme of myocardium leakage ( P <0 01), increase the content of NO in plasm ( P <0 01), reduce the level of ET ( P <0 01), and raise the ratio of NO to ET incidentally. Also, SAOH could obviously decrease the protein expression of Bax gene, but increase the protein expression of Bcl 2 gene. Conclusion SAOH could obviously protect myocardium during reperfusion injury, which might be associated with the increased content of NO, decreased level of ET in plasma, and reduced myocardial apoptosis.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期145-148,156,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省教育厅科研基金资助项目 (No. 98A0 76 )
