大鼠实验性根尖周病的酶组织化学研究

An Enzyme Histochemical Study of Rat Experimental Periapical Lesions

目的 :本研究旨在通过石蜡包埋的酶组织化学染色法 ,观察大鼠实验性根尖周病病损区成骨细胞、破骨细胞的活动情况。方法 :将大鼠右下颌第一磨牙开髓后暴露口腔 2～ 2 8d ,诱导根尖周病损形成 ;对其进行心内固定后 ,将含牙和牙槽骨的标本经EDTA脱钙 ,采用石蜡包埋法制作切片 ,进行酸性磷酸酶和碱性磷酸酶酶组织化学染色。结果 :石蜡切片经酶组织化学染色后 ,可清晰辨别出酶阳性的成骨细胞和破骨细胞。随根尖周病的发展 ,成骨细胞和破骨细胞在数量上呈一定规律性变化。结论 :石蜡切片的酶组织化学法可用于研究根尖周病损区骨细胞的行为变化。

Objective:The behaviors of osteoblasts and osteoclasts in the rat experimental periapical lesions were examined by using enzyme histochemical methods of paraffin-embedded bone tissues.Methods:The right mandibular first molar was exposed to the oral environment for 2～28d to induce the formation of periapical lesions; Each animal was fixed by an intracardiac perfusion of 4% paraformoldehyde in a solution of 0.05M phosphate buffer;The first molar parts were then demineralized with 10% EDTA, and after being made into paraffin sections,the enzyme histochemical stainings were given.Results:Osteoblasts with strong alkaline phosphatase activity and osteoclasts with acid phosphatase activity have been identified in the periapical lesions by light microscopy,and the changes of behavior of osteoblasts and osteoclasts have been investigated too during the different developmental periods of periapical lesions.Conclusions:The results suggest that enzyme histochemical applications to paraffin sections are a useful means for clarifying the behavior of bone cells in periapical lesions.