期刊文献+

扬子鳄胚胎中肾发生及退化 被引量:2

Development and degeneration of mesonephros in Alligator sinensis embryos
下载PDF
导出
摘要 目的:在11个不同发育时期扬子鳄胚胎中观察中肾的发生及退化过程。方法:采用电镜、细胞化学及免疫组织化学方法。结果:孵育第6期在中肾管前端附近出现一些中肾小泡。第8期形成“S”形中肾小管。第13~17期鳄胚体前部部分中肾小管盲端内陷,形成原始的肾小囊和肾血管球,中肾小管显著伸长并迂回曲折。第20~22期,体前后部中肾组织均已形成完整的肾单位。中肾小管颈段由纤毛柱状上皮细胞组成,近球小管上皮细胞含丰富的PAS阳性物质并呈表皮生长因子(EGF)、转化生长因子(TGFβ_1)和生长抑素(SS)免疫阳性反应。第24~28期,体前部至后部的中肾组织依次退化。结论:(1)扬子鳄中肾除重吸收作用外还具有内分泌功能;(2)中肾退化时,细胞凋亡依次表现为核固缩,出现大量凋亡小体,线粒体数目剧增并膨胀,线粒体等细胞器自溶及核消失;(3)中肾退化可能与小管细胞中TGFβ_1及SS大量表达有关。 Objective:To investigate the development and degenration of mesonephros in Alligator sinensis embryos at 11 different incubation stages. Methods:Electromicroscopic,cytocriemical and irnmunohistochernical methods were applied. Results:Some mesonephric vesicles appeared in the vicinity of the anterior of the mesonephric ducts at 6th stage. At 8th stage S-shaped mesonephric tubules took shape. During 13rd-17th stages a part of mesonephric tubuli in the anterior of the embryos invaginated at its blind ends, resulting in primitive renal sacs and glomerulus and mesonephric tubuli elongated distinctly and curved back and forth. All mesonephric tissue had produced integrated nephric units during 20th-22nd stages. The neck part of tubuli was covered with ciliated epithelial cells. The epithelial cells of proximal tubuli contained abound-ant PAS-positive substances and appeared epidermal growth factor(EGF), transforming growth factor pi (TGF Pi), so-matostatin immunoreaction. During 24th-28th stages mesonephric tissue from anterior to posterior of the embryos degen-eratd in turn. Conclusion: (1)Mesonephros of Alligator sinensis embryo,besides reabsortion,also has the function of endocrine. (2)While mesonephros degenerating,apoptosis takes place with following characteristics:nuclear condensation,appearance of many apoptosis bodies, rapid increase in the number and expending of mitochondria, self-decay of cell organs such as mitochondria and disappearance of nuclei. (3)Degeneration of mesonephros might be related with the excessive expression of TGF (3) and somatostain.
出处 《解剖学杂志》 CAS CSCD 北大核心 2003年第2期109-113,共5页 Chinese Journal of Anatomy
基金 国家自然科学基金(3947002) 安徽省自然科学基金(98241716)
关键词 扬子鳄 胚胎 中肾 发生 退化 分子机制 Alligator sinensis embryo mesonephros development degeneration
  • 相关文献

参考文献4

共引文献10

同被引文献24

  • 1韩雪梅,张作干.小白鼠胚后肾和生后肾发育的组织学和组织化学观察——核酸、多糖、琥珀酸脱氢酶[J].解剖学报,1983(3):318-321. 被引量:2
  • 2王仲涛,曹玉纯,吴淑兰,应国华,李相印,赵玉珍.对胎儿肾脏的研究 Ⅱ.冷冻复型电镜观察[J].解剖学报,1981(3):322-325. 被引量:2
  • 3陈玉琴,俞诗源,张虎林,张璟,尚建科,谢明仁.家鸡肾脏的研究进展[J].生物学通报,2007,42(4):3-5. 被引量:3
  • 4蒋唏东 薛社普.鸡胚发育图谱[M].北京:科学出版社,1983.124-128.
  • 5谭景和.脊椎动物比较胚胎学[M].哈尔滨:黑龙江教育出版社,1995.32.
  • 6Keller G,Lacaud G.Developmet of the hematopoietic system in the mouse.Exp Hematol,1999,27(5):777-787.
  • 7Johnson O W, Mugaas J N. Some histological features of avian kidneys. America Journal of Anatomy, 1970,127 : 423 - 436.
  • 8Mizuho O, Hirfumi S. The macula densa site of avian kidney. Cell and Tissue Research, 1971,120( 1 ) :29 - 36.
  • 9Robert F, Wideman Jr, Eldon J, et al. Microanatomy of the renal cortex in the domestic fowl. Journal of Morphology, 1981,168(3) :249 - 267.
  • 10Nicholson J K, Kendall M D. The fine structure of dark or intercalated cells from the distal and collecting tubules of avian kidneys. Journal of Morphology, 1983,136( 1 ) : 145 - 156.

引证文献2

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部