雌激素致癇作用部位的研究

The study of effective regions of estrogen in brain of convulsive rats

目的 了解雌激素在癫疒间 大鼠中枢神经系统的作用部位。方法 利用海人酸致疒间 和 6 氟二乙酯致疒间 的两种不同机制的癫疒间 模型 (单纯致疒间组 ) ,应用免疫组化法检测单纯致疒间 及给予雌激素后再致疒间 大鼠海马、大脑皮质、纹状体的FOS表达。结果 两种模型单纯致疒间 组海马、皮质、纹状体FOS表达较正常组显著增高 (均P <0 .0 1)。给予雌二醇 (E2 )后再致 疒间 ,海人酸模型中海马、皮质FOS表达较单纯致 疒间 组增加 (P <0 .0 1,P <0 .0 5 ) ;而 6 氟二乙酯模型中无变化。结论 在两种不同致疒间 机制的癫疒间 模型中 。

Objective To research the effective regions of estrogen in central nervous system of convulsive rats.Methods In two models of epilepsy with different mechanism induced by Kainic acid(KA) and flurothyl, we observed FOS expression of hippocampus, cerebral cortex and corpus striatum in the rats of convulsive group with and without estradiol (E 2) administration by means of immunohistochemistry.Results In the rats of convulsive group induced by KA and flurothyl, the FOS expression in hippocampus, cerebral cortex and corpus striatum was significant higher than those of normal control group (all P <0.01).In the rats of convulsive group induced by KA after E 2 administration,the FOS expression was increased evidently in hippocampus and cerebral cortex (respective P <0.01 and P <0.05) as compared with the convulsive group only induced by KA. Nevertheless , in the rats of convulsive group induced by flurothyl after E 2 administration,the FOS expression in hippocampus, cerebral cortex and corpus striatum was not increased (all P >0.05).Conclusion The FOS expression of rats'brains induced by E 2 is different in two models of epilepsy with different mechanism.