摘要
目的:建立一种多器官功能障碍综合征(MODS)合并急性肾衰竭(ARF)的动物模型。方法:12只健康杂交家猪随机分成实验组(E组)和假手术对照组(C组)。E组行盲肠结扎加穿孔(CLP),并加做双侧输尿管结扎术(UL)制作成MODS合并ARF的动物模型,C组行盲肠和双侧输尿管探查术。两组动物均予24 h静脉输液,观察6天中动物的生命体征及主要器官功能改变。结果:E组动物在CLP+UL后4 h直肠温度升高;术后72 h血清肌酐(Cr)、尿素氮(BUN)、K+、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平分别从术前的(91.40±10.85))μmol/L、(4.08±0.93)mmol/L、(3.81±0.57)mmol/L、(62.40±8.20)U/L、(53.00±14.85)U/L升高至(995.25±125.28)μmol/L、(48.80±1.41)mmol/L、(7.37±0.66)mmol/L、(115.75±33.42)U/L、(278.00±96.20)U/L,动脉血二氧化碳分压(PaCO2)从术前的(37.68±3.10)mmHg(1mmHg=0.133 kPa)升高至(51.98±4.51)mmHg(P均<0.01);pH值分别从术前的7.38±0.04下降至7.25±0.05(P<0.05);平均动脉压(MAP)从术后0 h的(160.20±18.58)mmHg降至术后72 h的(105.75±13.96)mmHg(P<0.01),6天内动物全部死亡,平均生存时间(74.96±23.00)h。C组全部存活。且上述指标基本正常。结论:盲肠结扎加穿孔造成严重腹腔感染致明显的多器官功能障?
Objective: To establish a composite pig model with multiple organ dysfunction syndrome (MODS) and acute renal failure (ARF). Methods: 12 pigs were randomly divided into two groups, group E received cecal ligation and puncture (CLP) plus bilateral ureteral ligation and group C received sham operation. The animals were infused with solutions containing glucose and sodium chloride for 6 days. Results: In group E, the rectal temperature increased 4 hours after operation. The indexes enhanced 72 hours after operation, including SCr from (91.40 ± 10. 85) μmol/L to (995. 25 ±125. 28)μmol/L, BUN from (4. 08 ± 0. 93) mmol/L to (48. 80±1.4l)mmol/L, serum kalium from (3.81 ±0.57)mmol/ L to (7. 37±0. 66)mmol/L, ALT from (62. 40 ±8. 20)U/L to (115. 75± 33. 42)U/L, AST from (53. 00 ±14. 85)U/L to (278. 00±96. 20)U/L and PaCO2 from (37. 68±3. 10)mmHg to (51.98±4.5l) mmHg ( P < 0. 01). The blood value of pH decrease from 7. 38±0. 04 to 7. 25±0. 05, ( P < 0. 05), and the value of MAP reduced from (160. 20 ± 18. 58) mmHg to (105. 75 ±13. 96)mmHg( P <0.01). The mortality in group E is 100% within 6 days after operation, and the survival time was (74. 96 ±23. 00)h. All the animals, survived in group C. Conclusion: The dysfunction in organs and systems was caused by severe intra-abdominal infection induced by CLP, meanwhile, acute anuric renal failure was produced by bilateral ureteral ligation. It was successful to produce the pig model with MODS and ARF.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2003年第3期196-199,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目(No 30170433)
关键词
肠穿孔
输尿管结扎
多器官功能障碍综合征
急性肾衰竭
猪
cecal puncture
ureteral ligation
multiple organ dysfunction syndrome
acute renal failure
pig