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酒精对大鼠背侧纹状体长时程突触抑制的影响 被引量:1

Effect of Alcohol on the Induction of Long-term Depression in the Dorsal Striatum in Rats
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摘要 目的:建立大鼠背侧纹状体长时程突触抑制(LTD)的诱导方法,并观察60 mmol/L酒精对大鼠背侧纹状体LTD诱导的影响,初步探讨酒精的作用机制。方法:制备纹状体冠状切面脑片,双极钨丝电极刺激皮层-纹状体传入通路,在大鼠背侧纹状体部位记录诱发的群峰电位(population spike,PS)。预先灌流60 mmol/L酒精及N-甲基-D-天冬氨酸(NMDA)受体阻断剂MK-801,再给予强直刺激。结果:对照组给予强直刺激后诱导出明显的LTD,PS幅值减小到原先的20%左右,并持续60 min以上;灌流酒精10 min再给予强直刺激,PS幅值出现先减小而后增加的变化,60 min时已经恢复到强直刺激前的80%;灌流MK-801后给予强直刺激阻断LTD的诱导。结论:强直刺激皮层-纹状体兴奋性传入通路可在背侧纹状体诱导明显的LTD,灌流酒精不影响LTD的产生,然而会影响LTD的保持,NMDA受体参与纹状体LTD的诱导。 Objective:To establish the method of the induction of long-term depression (LTD) in the dorsalstriatum of rats and to investigate the effect of alcohol on the synaptic plasticity in striatum. Methods: Coronary brain slices including striatum were prepared, a bipolar tungsten electrode was used to stimulate the cortex-striatum pathway and population spike (PS) in the dorsalstriatum area was recorded. Alcohol (60 mmol/L) and NMDA receptor antagonist MK-801 were pretreated before the delivery of high frequency stimulation (HFS) to investigate the mechanism of the effect of alcohol on the synaptic plasticity in the striatum. Results:In control slice,a significant LTD was found after HFS, and the PS amplitude was reduced to 20% of the value that showed before HFS and persisted at least 60 min. When the slice was pretreated with alcohol (60 mmol/L) 10 min before HFS there was still a reduction of the PS amplitude but it was recovered slowly to 80% of the control value in about an hour. Pretreatment of slice with MK-801 blocked the induction of LTD. Conclusion: NMDA receptor may play a key role in the induction of LTD in the striatum. Alcohol can not block LTD in the striatum but it will shorten the period of LTD induced by HFS.
出处 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2003年第3期207-209,共3页 Journal of Nanjing Medical University(Natural Sciences)
基金 江苏省教育厅自然科学基金资助项目(02KJB310001)
关键词 纹状体 长时程突触抑制 酒精 大鼠 striatum long-term depression alcohol rats
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  • 1袁孝如,中国药理学报,1996年,17卷,4期,278页
  • 2蔡南山,神经生物学,1992年,11页

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