新生猪缺氧缺血脑损伤模型制备的研究

Development of a Neonatal Piglet Model of Hypoxic Ischemic Brain Damage

目的研究新生猪缺氧缺血脑损伤(HIBD)模型的制备。方法生后7 d上海种白猪14只,双侧颈总动脉阻断,机械通气吸入低氧气体(FiO2 6％),缺氧缺血(hypoxia ischemia,HI)持续30 min。监测心率、温度、有创动脉血压、脑电图(EEG)、血气、血糖、乳酸等,进行神经行为评分及脑组织病理分析。结果与HI前比较,HI30 min时血糖、乳酸升高明显,心率、平均动脉压、PaO2、BE降低明显,HI 30 min EEG频率、电压均明显降低于HI前。HI后24 h和72 h神经行为评分明显低于HI前,脑皮层、海马和基底节HE染色病理积分分别为(2.4±0.6),(2.0±0.4),(2.0±0.7)。结论新生猪HIBD模型可操作性好,可以造成脑损伤程度较为一致的脑部病理变化,为研究新生儿窒息后缺氧缺血性脑病提供较可信的模型。

Objective To develop an improved neonatal piglet model of hypoxic ischemic brain damage (HIBD). Methods Fourteen 7-day-old piglets were subjected to temporary occlusion of both carotid arteries, followed by mechanical ventilation with low concentration of oxygen ( FiO2 = 0.06) for 30 minutes. Before and after the interventions, the heart rate, temperature, invasive blood pressure, blood gas, and concentrations of blood glucose and lactose were measured, electroencephalogram (EEG) was monitored, and neural behavior scores and pathologic changes of brain tissues were evaluated. Results Compared with the pre-intervention status, blood glucose and lactose concentrations significantly increased and the heart rate, mean arterial pressure, PaO2 and basic excess significantly decreased after the hypoxic-ischemic intervention. In addition, the frequency and voltage of EEG decreased 30 min after the intervention. The neural behavior scores were significantly lower at 24 h and 72 h after the intervention. The pathologic scores of the cortex, hippocampus and basal were 2.4 ± 0.6 and 2.0±0.4, 2.0± 0.7, respectively. Conclusions We have developed a reliable and reproducible piglet model of HIBD.