四色流式细胞术分析B细胞型急性淋巴细胞白血病免疫表型

Analysis of Immunophenotype of B-Lineage Acute Lymphoblastic Leukemia Cells by 4-Color Flow Cytometry

为了研究国人B细胞型急性淋巴细胞白血病 (B ALL)免疫表型特点 ,使用了四色流式细胞术CD4 5 /SSC设门分析 181例B ALL的免疫表型。研究结果发现 ,所有检测病例CD19阳性率 10 0 % ,HLA DR阳性率 98.9% ,CD38,CD10和CD34阳性率分别为 88.5 % ,76 .8%和 76 .8% ,CD117与T系抗原CD2和CD7在B ALL中很少表达。儿童组 (≤ 14岁 )CD10 + 比例较高 ,青少年 (15 - 18岁 )和成人组 (≥ 19岁 )髓系相关抗原CD13和 (或 )CD33表达较高。各年龄组CD10 + /CD34+ 型比例最高 ,随年龄增长CD10 -/CD34+ 型比例升高。CD10 -/CD34+ 型伴髓系抗原表达率明显高于其它亚型。与CD4 5 + 病例相比 ,CD4 5 -或者CD4 5 + / -病例常伴有较高的CD10表达。 4 3例标本RT PCR检测bcr/abl结果显示 :bcr/abl+ 组和bcr/abl-组伴髓系抗原表达率无显著性差异 ,m bcr/abl+ 主要见于CD10 + /CD34+ 型。结论 :典型的B ALL细胞表达CD19和HLA DR ,不表达CD117。不同发育阶段CD34,CD10和CD4 5表达不一。青少年组免疫表型与成人组相似。CD4 5 + 多见于CD10 -B ALL。儿童组髓系抗原表达率较低。m bcr/abl+ 多见于CD10 + /CD34+ 型B ALL。

To investigate the characteristics of immunophenotype of B lineage acute lymphoblastic leukemia (B ALL) cells in Chinese cases, B ALL cells from 181 patients were analyzed by 4 color flow cytometry with CD45/SSC gating. The results demonstrated that the antigen expression frequencies were as follows: CD19(100%), HLA DR(98.9%), CD38(88.5%), CD10(76.8%) and CD34(76.8%). CD117 and T antigens (CD2 and CD7) were rarely expressed. Childhood group (≤14 years) had the highest frequency of CD10. Adolescent (15-18 years) and adult groups (≥19 years) had a higher expression rate of myeloid antigens (CD13 and/or CD33). Subtype CD10 +/CD34 + took the highest percentage in three age groups. Percentages of CD10 -/CD34 + group increased by age. Subtype CD10 -/CD34 + had the highest myeloid antigen co expression. Comparing with CD45 positive cases, those with CD45 negative or CD45 +/- always had higher CD10 expression. Bcr/abl mRNA was evaluated in 43 samples from patients with B ALL. Myeloid antigen co expression was not different in bcr/abl + and bcr/abl - groups, and m bcr/abl + was mainly observed in subtype CD10 +/CD34 +. In conclusion, typical B ALL cells expressed CD19 +, HLA DR +, and CD117 -. CD34, CD10 and CD45 expression varied in different maturation stages. The immunophenotypic subtype of B ALL in adolescents was similar to that in the adults. Blasts with CD45 + were mostly seen in CD10 - cases. CD13 and/or CD33 co expression was fewer in children patients. m bcr/abl + was mainly seen in subtype CD10 +/CD34 +.