温度对胶原蛋白结构影响的二维红外相关光谱的研究

Two-dimensional correlation analysis of FTIR spectrum for collagen during variable temperature denaturing process

采用傅立叶红外光谱法 (FTIR)和二维相关分析 ( 2DCorrelationAnalysis)技术研究了胶原蛋白在加热 ( 15℃～ 95℃ )条件下构象变化的规律。结果表明 :二维相关分析能提供由温度变化引起的蛋白构象变化过程中分子结构动态变化的微观信息 。

The Fourier Transform Infrared Spectroscopy and Two Dimensional Correlation Analysis method were applied to study a denaturing process of collagen during varying temperature. It is shown by study results that the microcosmic information of a change in dynamic structure can be provided for collagen during denaturing process. An important theory reference was proposed to produce structural information of collagen and the mechanism of structural variation in the denaturing process