复方沙棘2号颗粒对大鼠局灶性脑缺血再灌注损伤的保护作用及机理探讨

Protection and mechanism of compound Hippophae rhamnoids L. gran ule 2 on focal cerebral ischemia-reperfusion in rats

目的 :研究复方沙棘 2号颗粒对大鼠局灶性脑缺血再灌注损伤的保护作用及作用机理。方法 :线栓法制作局灶性脑缺血再灌注模型。检测脑梗死体积比 ,血清MDA含量 ,血清SOD和GSH Px活性 ,并观察行为学改变。结果 :大鼠脑缺血再罐注后 ,血清MDA含量和脑梗死体积比显著增加 ,SOD和GSH Px活力明显下降。复方沙棘 2号可有效地防止上述改变。结论 :复方沙棘 2号颗粒对脑缺血再灌注损伤有显著保护作用。

AIM: To study the protection and mechanism of compound Hippophae rhamnoides L. granule 2 (CHRG2 ) on focal cerebral ischemia -reperfusion in rats. METHODS: Focal cerebral ischemia-reperfu sion model was made with suture-occluded method. The volume of cerebral infarct ion, the level of MDA in serum, the activities of SOD and GSH-Px in serum were measured. The behavior obstacles of rats were observed. RESULTS: After cerebral ischemia-reperfusion, the content of MDA in serum and the volum e of cerebral infarction increased significantly, the activities of SOD, and GSH -Px decreased significantly. CHRG2 could effectively prevent these changes. CONCLUSION: CHRG2 shows the protection against ischemia-reperfusio n injury, and the effect may be related to raising anti-peroxidation of the bra in tissue.