Ⅱ、Ⅲ组亲代型谷氨酸受体激动剂对6-羟基多巴诱导的PC12细胞毒性无保护作用

Lack effect of metabotropic glutamate receptors on neurotoxicit y of PC12 cells induced by 6-OHDA

目的 :阐明Ⅱ、Ⅲ组亲代谢型谷氨酸受体(metabotropicglutamatereceptors,mGluRs)激动剂对 6 羟基多巴 (6 hydroxydopamine,6 OHDA)诱导的PC1 2细胞毒性是否具有保护作用。方法 :应用高效液相色谱仪联用荧光检测技术测定谷氨酸浓度 ,应用四甲基偶氮唑盐 (MTT)比色法测定PC1 2细胞活性。结果 :6 OHDA剂量依赖性地诱导PC1 2细胞释放谷氨酸、减低细胞活性 ,Ⅱ组mGluRs激动剂DCG IV和Ⅲ组mGluRs激动剂L AP4对 6 OHDA诱导的PC1 2细胞释放谷氨酸和细胞活性的降低均无显著影响。结论 :6 OHDA对多巴胺神经元的损伤作用与其诱导谷氨酸过度释放及其继发的兴奋性神经毒性有关 ,Ⅱ、Ⅲ组亲代谢型谷氨酸受体激动剂对 6 OHDA诱导的PC1

AIM: To explore if metabotropic glutamate receptors (mGluRs) i nduces neuroprotection against 6-hydroxydopamine (6-OHDA) neurotoxicity in PC1 2 cells. METHODS: The alteration of the glutamate in extracellul ar fluid of PC12 cells was detected by high performance liquid chromatography (H PLC) with a fluorescent detector. The cytotoxic activity of PC12 cells was assay ed by means of MTT colorimetric method. RESULTS: 6-OHDA dose-d ependently increased glutamate release and decreased cell activity of PC12 cells , and the mGluRs ligands had no significant effect on that. CONCLUSION: The mGluR3 ligands has no protective effect on neurotoxicity of PC12 cel ls induced by 6-OHDA.