石菖蒲及其有效成分对小鼠学习记忆的作用及α-细辛醚药物动力学研究

Functions of Acrous Gramimeus and Its Effective Constituents on Learning and Memory of Mouse and Study on Pharmacodynamics ofα-asarone

采用不同的动物实验方法,用石菖蒲去油煎剂(Ⅰ)、总挥发油(Ⅱ)、α-细辛醚(Ⅲ)进行实验。结果表明:对正常小鼠学习和记忆获得能力(跳台法试验),戊巴比妥钠造成的记忆获得障碍模型(电迷宫法试验),亚硝酸钠造成的记忆巩固不良模型(跳台法试验),乙醇造成的记忆再现缺失模型(跳台法试验)均有不同程度的促进作用和改善作用。测定其主要有效成分α-细辛醚在大鼠体内各项药物动力学参数,结果表明:α-细辛醚口服吸收快,在大鼠体内分布广泛。以上实验结果还表明:石菖蒲对学习记忆作用的主要有效部位是总挥发油(Ⅱ),其中α-细辛醚(Ⅲ)是其主要有效成分,去油煎剂(Ⅰ)有一定作用,但作用较弱。

Experiments have been carried out with the extracts(Distlled oil is not included.),total volative oil andα-asarone of Acrous Gramineus through different animal experiments and their results have showed different functions in the promotion and improvement of the learning and memory of normal mouse,such as the increase of the ability of its learning and memory(by step-down test),the formation of a barrier model made by pento in its memory(by the experiment of electric maze method),the imperferct model made by sodium nitrite in the consolidation of its memory(by the experiment of diving tower method)and the reappearance of the model of ntobarbita barbitalum natricum in its memory(by electric maze test),the generation of a imperferct model made by sodium nitrite in the consolidation of its memory(by step-down test)and the reappearance of the model of its amnesia made by ethane(by electric maze test).And various pharmacodynamical parameters ofα-asarone,a main effective constituent of Acrous Gramineus,in the body of rat have also been tested and the result has indicated thatα-asarone taken orally can be absorbed quickly and distributed widely.All the main functions of Acrous Gramineas on learning and memory are located in its total volativeoil,of whichα-asarone forms its most effective constuent,while the functions of its extracts are relatively weak.