广谱晚癌康对人肝癌细胞凋亡相关基因表达的影响

EFFECT OF EURYTOPICITY WAN′AIKANG ON APOPTOPIC GENE EXPRESSION INHUMAN PRIMARY HEPATOMA CELLS

目的 :探讨广谱晚癌康 (Ⅰ号、Ⅱ号 )治疗原发性肝癌的作用机制。方法 :选择生长良好的人肝癌细胞株 ,分别培养实验组和对照组的肿瘤细胞 ,滴加稀释度为 1:5 0抗人bcl- 2、Bax和Fas及PCNA的单克隆抗体 ,采用荧光免疫组化显示技术和激光共聚焦显微细胞仪的定量技术 (Confobalmicroscope,ACAS5 70型 )观测扫描细胞的荧光值。结果 :广谱晚癌康Ⅰ号及Ⅰ号 +Ⅱ号能影响人肝癌细胞凋亡相关基因Bcl-2、Bax、Fas表达增加 ,Ⅱ号能影响Bcl- 2表达减少 ,而影响Fas表达增加。结论 :广谱晚癌康 (Ⅰ号、Ⅱ号 )对人体肝癌细胞有治疗作用 ,其作用机制可能是通过对凋亡相关基因调控的表达来实现的。同时也表明广谱晚癌康的抗癌作用 ,Ⅰ号、Ⅱ号联合作用 >Ⅰ号单纯使用 >Ⅱ号单纯使用。

Objective:To discuss the mechanism of eurytopicity wan′aikang on primary hepatic cancer.Methods:Living tumor cells reaction to eurytopicity wan′aikang was detected by immunofluorescent method and laser microprobe cytofluorometry method.Results:The increment of Bcl-2,Bax and Fax gene expressions were observed following eurytopicity wan′aikang No.1 and No.1+No.2 treatment.The inhibition of Bcl-2 gene expression and the increment of fas gene expression were observed following eurytopicity wan′aikang No.2 treatment.Conclusion:Eurytopicity wan′aikang can effect the expression of the related genes in human hepatoma cells.And it also shows that the treatmental effects are followed by the sequence:eurytopicity wan′aikang No.1+No.2>eurytopicity wan′aikang No.1>eurytopicity wan′aikang No.2