大肠癌p53、K-ras基因突变研究

Study of p53 and K-ras gene mutation in colorectal carcinoma by PCR-SSCP

目的探讨p53、K-ras基因突变与大肠癌发生、发展的关系.方法应用PCR-SSCP方法研究68例大肠癌和癌旁组织以及20例正常组织p53、K-ras基因突变情况.结果大肠癌组中p53、K-ras基因突变率分别为47.1％和44.1％,明显高于癌旁组(分别为13.2％和7.4％).20例正常组织中未检出p53、K-ras基因突变.大肠癌伴有淋巴结转移及远处转移者。p53、K-ras基因突变率明显高于无淋巴结及远处转移者;p53、K-ras基因突变与组织学分型无关.结论 p53、K-ras基因突变与大肠癌发生、发展有密切关系,在细胞癌变中起重要作用,可作为评估大肠癌转移的分子生物学指标.

Objective To study the relation between p53, K-ras gene mutation and the occurrence and development of colorectal carcinoma. Methods The positive rate of p53 and K-ras gene mutation in 68 cases cancer tissue, tissue sampces adjacent to tumors and 20 normal controls were studied by PCR-SSCP. Results The positive rate of p53 and K-ras gene mutation in cancer tissue (47.1%, 32/68; 44.1%. 30/68) were significantly higher than in tissue adjacent to tumur, (13.2%. 9/68; 7.4%. 5/68). p53, K-ras gene mutation were not detected in 20 normal tissue. The positivity of p53 and K-ras gene mutation in the group with lymph nodes and distant metastasis was markedly higher than those in the group without metastasis, but their relation to the histologic types was not significant Conclusions A close correlation was present between p53, K-ras gene mutation and the occurrence and development of colorectal carcinoma. p53 and K-ras gene mutation in cancer tissue may play an important role during colorectal carcinogenesis, and could be regarded as biomolecular index for the evaluation of metastasis in colorectal carcinoma.