微囊化转mIFN-γ基因CHO细胞皮下移植促进荷瘤小鼠T_H1T_H2的漂移

Influence of microencapsulated CHO cells translated mIFN-γ on T_H1/T_H2 in tumor burden mice

目的 通过观察微囊化转mIFN γ基因CHO细胞皮下移植对荷瘤小鼠TH1 TH2类细胞因子的影响 ,进一步了解外源性IFN γ对TH1 TH2平衡的调节作用 ,同时验证微囊化基因工程细胞移植治疗恶性肿瘤的可行性。方法 将mIFN γ基因转染到正常细胞CHO ,然后进行微囊化 ,移植到荷瘤小鼠的皮下 ,2周后 ,测定小鼠血清TH1和TH2类细胞因子IFN γ、IL 2、IL 12、IL 4、IL 10的水平 ,并与对照组相比较。同时测量肿瘤的平均直径 ,以观察肿瘤的生长速度 ,并观察荷瘤小鼠的生存期。结果 经微囊化转mIFN γ基因CHO细胞皮下移植干预治疗后 ,小鼠血清TH1类细胞因子IFN γ、IL 2、IL 12水平明显升高 ,而TH2类细胞因子IL 4、IL 10变化不明显 ;小鼠肿瘤的生长速度明显减慢 ,生存期明显延长。结论 外源性IFN γ促进荷瘤小鼠TH1细胞的分化 ,使TH1 TH2向着TH1方向漂移 ;微囊化基因工程细胞的移植 ,有望替代基因工程药物 ,成为治疗恶性肿瘤的又一新平台。

Objective To study the influence of transplantation of microencapsulated CHO cells translated IFN-γ gene on T H1/T H2 type cytokines, and adjustment of foreign IFN-γ to T H1/T H2 cells balance. The feasibility of microencapsulated genetic cells as tumor-killer was studied. Methods The mIFN-γ gene was translated into CHO cells. After microencapsulated, the CHO cells translated IFN-γ gene were transplanted into tumor burden mice. After 14 days, the level of serum IFN-γ, IL-2, IL-12, IL-4, IL-10 was examined. The tumor size and survival time was studied also. Results The level of serum IFN-γ, IL-2, IL-12 was significantly higher, but the change of IL-4, IL-10 were not significantly. The speed of tumor growth was significantly slowed down. The survival time was elongated. Conclusions The foreign IFN-γ promotes T H1 cells differentiation, T H1/T H2 move to T H1. The microencapsulated genetic enginnering cells may be a new candidate for cancer treatment as an alternative of genetic enginnering drugs. [