PPN抑制HBV-DNA表达及复制的体外实验

Effects of PPN on Duplication of HBV in 2.2.15 Cell Strain

目的 体外观察PPN对HBV DNA表达、复制的抑制效果及其细胞毒性作用。方法 取 10 μg/ml、2 0 μg/ml、 4 0 μg/ml、 80 μg/ml、 16 0 μg/mlPPN水溶液分别加入 2 2 15细胞株培养悬液中 ,8天后用放射免疫法检测上清液中HBeAg的含量 ,并计算HBeAg抑制率、细胞存活率、HBeAg抑制率为 5 0 %时的药物浓度 (ID50 )、实验组存活细胞为对照组的 5 0 %时的药物浓度 (CD50 )和治疗指数 (TI)。PCR技术检测HBV DNA阳性血清中加入 16 0 μg/mlPPN水溶液后对HBV DNA复制的抑制作用。 结果 当加入的PPN浓度为 16 0 μg/ml和 80 μg/ml时 ,HBeAg抑制率分别为 89 8%和 82 4 % ,细胞存活率分别为 98 7%和 99 2 % ;HBV DNA阳性血清中加入PPN后可有效抑制其复制。结论 PPN可有效抑制HBV DNA的表达和复制 ,且对靶细胞 (肝细胞 )无细胞毒作用 。

Objective To explore effects of PPN on duplication of Hepatitis B virus and it's cytotoxicity to 2 2 15 cell strain. Methods 10μg/ml,20μg/ml,40μg/ml,80μg/ml,160μg/ml PPN water solution were respectively added into cell culture fluid of 2 2 15 cell strain and HBeAg in supernatant layer was assayed by Polymerase chain reaction(PCR).Inhibitive rate of HBeAg,cellar survival rate,the drug concentration were calculated(ID 50 ).When inhibitive rate of HBeAg is 50% as well as the drug concentration(CD 50 )and therapeutic index(TI)when cell in experimental test group was half of that in control group. Results When drug concentration of PPN is 160μg/ml,80μg/ml,inhibitive rates of HBeAg were 89 8%and 82 4%,cellar survival rates were 98 7%and 99 2%respectively.Conclusion PPN performed an organic function to inhibit duplication of Hepatitis B Virus in Vitro,and it wasn't cytotoxic PPN should be studied further and applied extensively.