luxAB基因标记甲基对硫磷降解菌DLL-1在土壤和植株根部的生态行为研究

Ecological behaviors of luxAB-labeled parathion-methyl-degrading strain DLL-1 in rhizosphere of plant.

发光酶标记是 1种有效的跟踪微生物在生态环境中动态行为的技术手段。采用luxAB基因标记技术对甲基对硫磷降解菌DLL - 1在土壤中的分布和植株内的定殖情况进行了研究。结果表明 ,DLL - 1可以较长时间的在植株根际定殖 ,3 0d后仍可用X -感光片检测到菌体的存在 ,而根际外未检测到DLL - 1。植株根剖开后在培养基上培养 2 4h后进行X -光片曝光 ,发现DLL - 1能够进入植株根内并定殖。菌株回收后采用测定其农药降解活力和检查质粒图谱 2种方法证实 ,所观测的回收菌株就是接种的DLL -

Luciferase labeling is a powerful tool for tracing dynamic activity of microorganisms in the ecosystem.Experiments were conducted with DLL-1 that was labeled with luxAB to investigate distribution of the pesticide-degrading bacterium in soil and its invasion into roots.Luminescence could be detected even 30 days after labeling.The roots were dissected and cultured on plate for 24 hours and then exposed to X-ray film.Results also showed that the strain could invade into root of the plant.Parathion hydrolysis and plasmids detection also verified colonization and invasion of the bacteria of the strain.