摘要
目的:进一步了解乙肝产妇的母乳中HBV DNA含量及其传染性,更好地指导母乳喂养。方法:利用荧光实时定量PCR检测技术,对45例乙肝血清学阳性的产妇进行血浆及乳汁中HBV DNA的定量检测。结果:45例产妇血浆和乳汁中HBV DNA的检出率分别为73%(33/45)和67%(30/45),其HBV DNA含量的对数值具有良好的相关性,(r=0.837,n=45,P<0.01).HBeAg(+)与HBeAb(+)两组间比较血浆及乳汁HBV DNA的含量,两者存在显著性差异(P<0.01)。HBeAg(+)组含量高于HBeAb(+)组。结论:乳汁与血浆中HBV DNA含量具有较好的相关性,血浆中HBV DNA高拷贝的产妇最好不要进行母乳喂养。产妇血浆中HBeAg与乳汁及血浆中HBV DNA含量都具有明显关系,它的存在提示血浆及乳汁中的HBV DNA含量较高。
objetive: In order to conduct breast feeding more scientificly, we proceeded a study on the HBV DNA content and infectivity in HBV serum markers positive parturients. Methods: To detect HBV DNA by the fluorescent quantitative PCR. Results: HBV DNA positive ratio were 73% and 67% respectively in the plasm and breast milk of the 45 parturients. The HBV DNA content in the breast milk was found to be highly related to the plasm (r=0.837, P<0.01), HBV DNA quantity in the plasm and breast milk in HBeAg positive parturients were significantly higher than that in the HBeAb positive parturients. (P<0.01). Conclutions: There was a relationships about the HBV DNA concentration between the breast milk and the plasm. The presence of HBeAg in the plasm indicated that the HBV DNA quantity in both the plasm and the breast milk were high. It would be better for the parturients with high HBV DNA quantity in the Plasm or in the breast milk to choose manual feeding.
