基因工程菌强化芳香化合物的处理工艺

The treatment technique for augmentation of aromatic compounds by genetic bacterial strains

分别固定克隆有甲苯加双氧酶基因的工程菌和筛选出的野生菌株,串联两种固定化细胞反应器,研究以基因工程菌突破关键步骤的限制,筛选菌株辅助完成彻底降解芳香类污染物复合工艺可行性和强化效果.克隆有苯降解过程中的关键基因——甲苯加双氧酶的基因工程菌E. coli. JM109(pKST11)对苯具有较高的降解效率和降解速度,应用于固定化细胞反应器中效果突出.在较短的水力停留时间内,可以将1500mg/L苯降解70%,降解速度为1.11mg/(Ls),延长水力停留时间,可以使去除率达到95%以上.该反应器对高浓度的苯具有突出的处理效果.同时所得到的产物为环己二烯双醇,可以被野生非高效菌W3快速利用.

With immobilizing respectively the genetic bacterial strain E. coli JM109 (pKST11) and the wild strain to make contacting reactors, the feasibility and the augmentation effect of the combined technique of bacteria strain selected to assist degrading aromatic pollutant thoroughly, and also those of genetic bacterial strain to break through key process were studied. E. coli. JM109 (pKST11) with the key gene of the toluene degrading process possessed rather great degradation efficiency and velocity of benzene, especially outstanding in immobilized cells reactor. 70% of benzene of 1500mg/L could be degradated in rather short hydraulic retention time (HRT) and the degradation velocity is 1.11mg/(Ls). The removal rate could be above 95% with increase of HRT. This reactor possessed outstanding treatment efficiency on benzene of high concentration, meanwhile the produce cis-diol obtained could be utilized rapidly by wild non-efficient bacterium train W3.