白细胞介素-15对脐血和成人外周血单个核细胞抗肿瘤活性的影响

The effect of rhIL-15 on the antitumor activity of cord blood and peripheral blood mononuclear cell

目的 :探讨白细胞介素 15 (IL 15 )对脐血单个核细胞 (CBMNC)和成人外周血单个核细胞 (PBMNC)抗肿瘤活性的影响。方法 :采用MTT法分别检测静止的CBMNC和PBMNC对K5 6 2和HL 6 0细胞株的杀伤活性、经不同浓度的IL 15及不同效靶比条件下激活的CBMNC和PBMNC抗瘤活性 ,采用ELISA法测定培养上清中IFN r及TNF a水平 ,采用APAAP检测CD56、CD16表面抗原。结果 :①静止CBMNC抗肿瘤活性显著低于PBMNC ,经IL 15活化后 ,脐血NK活性显著增强 ,可达成人水平 ,脐血LAK活性显著高于成人血LAK活性。②活化后培养上清中的IFN r及TNF a水平均显著增强 ,但脐血培养上清中两种细胞因子的水平仍低于成人血。③活化前后脐血NK细胞CD56表达显著增强。结论 :IL 15能显著提高CBMNC的抗肿瘤活性 ,为今后优选IL

Objective:IL 15 was recently identified as a novel cytokine that shares many biologic properties with IL 2, our investigation was to study the effect of IL 15 on cord blood mononuclear cell (CBMNC) and peripheral blood mononuclear (PBMNC) .Method:CBMNC and PBMNC incubated with IL 15 at different concentration were cocultured with K562(NK sensitive) or HL 60 (NK resistant) at different ratio of effect/target. By using MTT assay to test the specific killing of cord blood and peripherl blood,using ELISA to test the interferon r (IFN r) and tumor necrosis factor a (TNF a) protein levels and using APAAP to assay the expression of CD 56 and CD 16 antigens.Result:①Anactivited citoxicity of fresh CBMNC was significantly lower than that of PBMNC.When incubated with IL 15,the level of CB NK cyoxicity was significantly increased and even could reach the level of PBMNC,the level of CB LAK citoxicity was significantly higher than PB LAK.②IL 15 also significantly induced IFN r and TNF a protein production.③When incubated with IL 15 theCD 56 antigen of CB NK was significantly increased witch suggest IL 15 might increase the cyoxicity of CBMNC by increasing the number of CD 56 NK cells in cord blood.Conclusion:IL 15 can significantly increase the cyoxicity of CBMNC.Our study suggests that IL 15 can be an alternative cytokine for the adoptive immunotherapy of malignant and nonmalignant disorders.