蒽酮-硫酸比色法测定麦冬多糖的含量

Determination of Pachyman in Radix Ophiogonis content by Anthrone-Sulphuric Acid Colrimetry

目的 建立麦冬多糖的含量测定方法。方法 采用蒽酮 -硫酸比色法。结果 6 2 6nm处有最大吸收峰 ,线性范围在2 5 6～ 2 5 6mg·L-1,平均回收率为 10 2 4 6 % (n =4 ,RSD =2 0 2 % ) ,麦冬多糖精制品的含量为 6 0 %以上。结论 该方法快速准确 ,重现性好 。

AIM To establish a simple and accurate mothod for the determination of pachyman in Radix Ophiogonis. METHOD Anthrone Sulphuric Acid colrimetry was used. RESULTS max=626 nm, and standard curve was liner in the concentration range 2 56～25 6 mg·L -1 ,the average recovery was 102 46%,RSD=2 02%. CONCLUSION The method was simple,rapid and reliable.