雌激素对去势大鼠骨骼中微量元素的影响

Influence of estrogen on trace elements in the bone of emasculated rat

目的 通过去卵巢的大鼠形成的骨质疏松症模型来探讨微量元素Cu、Mn、V、F、Mo、Co、Fe、Zn、Cr、Ba、B、Sr、Pb、Cd和Mg、Ca、P等元素在骨骼中的含量变化。方法 先用11个月龄雌性SD大鼠39只,随机抽样分成3组。A组为正常对照组,假手术处理;其余大鼠均予摘除双侧卵巢。3个月后,将摘除卵巢术后生存的大鼠标记后,随机分为2组,B组为模型组,C组为尼尔雌醇治疗组。3个月后处死大鼠,用DEXA检测双侧胫骨干骺端骨密度后,将右胫骨进行微量元素检测。结果(1)各组大鼠在左、右侧胫骨干骺端处的骨密度和骨矿含量均差异有显著意义,其中模型组最低。(2)模型组(B组)大鼠与假手术组(A组)比较,大鼠骨骼中Fe、Zn、Cu、Mn、V、Sr(P<0.01)和Mo、B、Co、Cr、F、Cd、Mg、Ca、P(P<0.05)等的含量下降;而Ni、Ba、B、Pb等的含量变化差异无显著意义。(3)雌激素治疗组(C组)中,与假手术组(A组)比较,雌激素治疗组大鼠骨骼中Ca的含量较低(P<0.05),而F的含量上升(P<0.05);而与模型组(B组)比较,Cu、Mn、V、Fe、Cd、Sr、Zn、Co(P<0.01)和B、Cr、Mo、F、Mg、Ca、P(P<0.05)等元素在雌激素治疗组的大鼠胫骨中的含量上升;而Pb、Ba、Ni等的含量变化差异无显著意义。(4)在各个组别中,Ni、Pb、Ba的含量在骨骼中的变化差异无显著意义。结论 骨骼中大?

Objective Ovariectomized rats were used as the model of postmenopausal osteoporosis to investigate the content changes of trace elements including Cu, Mn, V, F, Mo, Co, Fe, Zn, Cr, Ba, B, Sr, Pb, Cd, P in bones of the model rats.Method Thirty - nine SD rats of 11 month old were randomly divided into 3 groups, 13 rats in each group. Group A as control group was operated without extirpating ovary and only making an incision. All of the rest rats were ovariectomized. The survived ovariectomized rats were marked 3 months later and randomly divided into two groups: group B as model group and group C as treatment group. The rats were sacrificed after another 3 months. The right tibiae of rats were harvested after bone densities of metaphyses of bilateral tibiae were measured. The contents of trace elements in bones of the rats were then detected using an atomic absorption spectrophotometer. Results ( 1) There were significant differences in bone densities or bone mine contents of metaphyses of bilateral tibiae among each group of rats. The above value in group B was the lowest among all other groups. (2) The values of Fe, Zn, Cu, Mn, V, Sr in bones of rats were lower in group B than those in group A ( P < 0.01) , and so were Mo, B, Co, Cr, F, Cd, Mg, Ca and P ( P < 0.05 ) . There were no significant differences in the content changes of Ni, Ba, B and Pb between two groups. ( 3 ) Comparing to the group A, the content of Ca was lower in the group C(P < 0.05) , on the contrary, the content of F increased ( P < 0.05) . Comparing to the group B, element contents of the tibiae in rats including Cu, Mn, V, Cr, Fe, Cd, Sr, Zn and Co increased in the group C(P <0.01) . So did B,Cr,Mo,F,Mg,Ca and P (P <0.05) . There were no significant differences in the content changes of Pb, Ba, and Ni between two groups. (4) As to the content changes of Ni, Pb, and Ba in bones, there were no significant differences in each group. Conclusion The content changes of most trace elements in bone are closely associated with estrogen levels. Estrogen improves these trace elements deposition in bone by means of influencing on metabolism of bone cell, thereby the change of bone density.