藏药五脉绿绒蒿中的生物碱

Alkaloids from a Tibetan medicine Meconopsis quintuplinervia Regel

目的 研究藏药五脉绿绒蒿 (MeconopsisquintuplinerviaRegel)的化学成分。方法 用多种色谱技术进行分离纯化 ,用IR ,MS ,1D 和 2D NMR鉴定化合物结构。结果 从 95 %乙醇提取物中分离出 3个生物碱 ,分别鉴定为去甲血根碱 (norsanguinarine ,I)、O 甲基淡黄巴豆亭碱 (O methylflavinantine ,II)和五脉绿绒蒿碱 (meconoquintupline ,III)。结论 化合物III为新化合物。

Aim To reinvestigate the chemical constituents of the ethanolic extract of Meconopsis quintuplinervia Regel which is a traditional Tibetan medicine used for treatments of hepatitis, tuberculosis etc .. Methods The compounds were enriched by column chromatography techniques over silica gel, macro porous resin and Sephadex LH 20 absorbents, and finally purified by reverse phase preparative HPLC methods with isocratic mobile phase systems of methanol H 2O acetic acid (500∶500∶1) and acetonitrile H 2O acetic acid (200∶800∶1). Structural determination of the pure compounds were based on extensive analyses of modern spectroscopic methods including IR, MS, HRMS, 1D and 2D NMR spectra. Results Three alkaloids were obtained and their structures were elucidated as norsanguinarine (I), O methylflavinantine (II) and 6 methoxy 17 methyl 2,3 [methylenebis(oxy)] morphin 5 en 7 one (III). Conclusion Norsanguinarine (I) was isolated from genus Meconopsis for the first time, and 6 methoxy 17 methyl 2,3 [methylenebis(oxy)] morphin 5 en 7 one (III) is a new alkaloid named as meconoquintupline.