广西壮族人群载脂蛋白B基因限制性片段长度多态性分析

RFLP of ApoB gene in Guangxi Zhuangzu and Hanzu population

目的 :探索广西地区壮、汉两族人群ApoB基因多态性的分布特点及其与冠心病、脑梗塞发病之间的关系。方法 :应用聚合酶链反应 (PCR)对广西壮族 17名冠心病患者 (CHD)、14名动脉粥样硬化性脑梗塞患者 (ACI)和 62名健康人的ApoB基因的XbaI、EcoRI位点的限制性片段长度多态性 (RFLP)进行了研究。结果 :无论是广西壮族人群冠心病组、脑梗塞组与正常对照组在XbaI位点以X- X- 为主要基因型 ,少见X+ X+ 、X+ X- 基因型 ;在EcoRI位点以E+ E+ 为主要基因型 ,少见E+ E- 、E- E- 基因型。X+ 等位基因在壮族三组人群中的频率分别为 :0 117、0 0 714、0 0 4 92 ,两两之间没有显著性差异 (P >0 0 5 ) ;E-在广西壮族三组人群中的频率为 :0 0 3 85、0 10 7、0 0 4 84 ,两两之间的差异未达统计学意义 (P >0 0 5 )。结论 :广西壮族人群冠心病、脑梗塞的发病与XbaI。

Objective:To research the relationship between the polymorphisms of the apoliprotein B gene and coronary heart disease(CHD) and the relationship between the polymorphisms of the apoliprotein B gene and atherosclerotic cerebral infarction(ACI) in Guangxi Zhuangzu and Hanzu population.Methods:Xba I and EcoR I allele frequencies of restriction fragment length polymorphisms(RFLPs) of the ApoB gene in 17 Guangxi Zhuangzu CHD patients,14 ACI patients and 62 healthy subjects were studied by means of polymerase chain reaction(PCR).Results:For Xba I polymorphic site,X -X - was major genotype in the three groups,and X +X -?X +X + genotypes were rarely found.For EcoR I polymorphic site,the major genotype was E +E + but E +E - and E -E - genotypes were rarely found.The frequencies of X + and E - alleles in the three groups was 0 117?0 0714?0 0492 and 0 0385?0 107?0 0484,respectively.The allelic frequencies did not differ significantly between any two groups.Conclusion:The study suggests that there is no significant association between the Xba I or EcoR I allelic distribution and the development of CHD and ACI in Guangxi Zhuangzu population.