种子细胞库的建立及应用

Our Cell Bank and Its Application

目的 用细胞和生物材料体外培养、体内移植 ,修复骨及软骨的缺损。检测新药、新材料毒性实验 ,需要不同类型的种子细胞。为了防止细胞老化和污染 ,让实验具有科学性和可重复性 ,特别是软骨细胞培养 ,建立细胞库更有必要。方法 无菌取目的组织 ,用胶原酶、胰蛋白酶加纱巾法原代培养 ,建立具有增殖能力强 ,无污染 ,长期冻存 ,复苏成功率高的细胞系。结果 自建骨、骨膜、软骨、骨髓、骺板软骨等 2 0个细胞系。还收集了其他细胞系 16个。为院内外 35名研究生 ,98次复苏冻存细胞均获成功。结论 原代培养用酶消化加纱巾法分离成功率高。 2 2次原代培养 ,2 0次成功建系。建立种子细胞库为科研教学提供了方便。既省时 ,又节约经费 。

Objective Seeding cell is one of the foundation elements in tissue engineering.Different study,such as co culture of new material and cell,cell transplantation,bone and cartilage repair and new medicine trial,need different cells.The cell bank can save a lot of time and money in first generation cell harvesting and culturing,preventing the aging of cell and pollution,promoting the reproducibility,especially important for chondrocyte.Methods Harvest the cell from enzymic (collagenase and trypsase) dissolved tissue chips,culture in small nylon pitch,store in liquid nitrogen and proliferation in any time.Results Sixteen seeding cells from bone,periosteum,cartilage,bone marrow,epiphysis and etc.The rewarmed and regenerated cells were employed by 35 graduated degree student for 98 studies successfully.Conclusion Our way of harvesting,culturing,storing and regenerating of the seeding cells is practically applicable,it is highly comparable and reproducible.