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温度对CP43内色素分子间能量传递的影响 被引量:1

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摘要 对CP43进行不同温度处理5分钟,采用锁模Ar^+激光器输出的514.5nm的皮秒光脉冲作为激励光,通过探测CP43的荧光光谱特性,来研究色素分子间的能量传递。分析表明,20℃处理后,CP43内Chla671向Chla679和Chla682同时传递能量,并且Chla679也向Chla682传递能量,Chla682获得的能量是Chla679获得能量的1.5倍。42℃处理后,Chla671向Chla679和Chla679向Chla682的能量传递加速,最终能量几乎全部由Chla682接收。48℃处理后,Chla679向Chla682的能量传递减慢,甚至断裂,Chla671将能量分别传递给Chla679和Chla682,但是Chla682接收到的能量略多于Chla679色素分子。60℃处理后,造成了Chla671向Chla679能量传递截止,Chla671向Chla682的能量传递发生了部分截止,因此Chla671的能量部分传递Chla682。不同温度处理后的荧光强度变化表明,Chla671接收到的能量受到蛋白空间构象的影响,在48℃处理后,接收到的能量是最多的,60℃处理后,接收到的能量最少。
出处 《光子学报》 EI CAS CSCD 北大核心 2002年第0Z2期123-128,共6页 Acta Photonica Sinica
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