应用组织微阵列和原位杂交检测卵巢上皮性肿瘤中HIF-1α mRNA的表达

Tissue microarray and in situ hybridization study on hypoxia inducible factor 1α mRNA expression in epithelial ovarian tumor

目的 探讨缺氧诱导因子 1α(HIF 1α)在卵巢上皮性肿瘤中的表达及意义。方法 采用组织微阵列 (TMA)新技术和原位杂交方法 ,回顾性研究 2 95例卵巢上皮性肿瘤中HIF 1αmRNA的表达情况 ,并以 13例正常卵巢组织作对照。结果 正常卵巢、卵巢上皮性良性肿瘤、交界性肿瘤和卵巢癌组织的HIF 1αmRNA表达阳性率分别为 0、13.2 %、4 2 .1%和 81.9%。交界性肿瘤和卵巢癌组织中的HIF 1αmRNA表达显著高于正常卵巢和良性卵巢上皮性肿瘤 (P <0 .0 0 1) ,卵巢癌显著高于交界性肿瘤 (P <0 .0 0 1)。卵巢癌组织中HIF 1αmRNA的表达与临床病理分期和病理类型无相关性 (P >0 .0 5 )。但在不同组织学分级 (即高、中、低分化 )的卵巢癌组织中 ,HIF 1α表达率分别为 6 0 .4 %、81.2 %和 96 .4 % ,与组织学分化程度呈负相关 (P <0 .0 0 1)。结论 HIF 1α在卵巢癌的发生、发展过程中可能起着重要作用。组织微阵列技术适用于大样本量组织的检测 ,是一种高效、快速、可比性强的分子生物学研究方法。

Objective To study the expression and significance of hypoxia inducible factor 1α (HIF 1α) in epithelial ovarian tumors. Methods The expression of HIF 1α mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples. Results The expression rates of HIF 1α mRNA were 0, 13.2% ?42.1% and 81.9% in normal ovarian tissue, benign, borderline and malignant ovarian tumors. Expression rate of HIF 1α mRNA in borderline and invasive tumor was significantly higher than those in normal ovarian tissue and benign tumor ( P <0.001). Statistical analysis revealed that the expression of HIF 1α mRNA was not related to FIGO stages or histological subtypes. Close negative relation was observed between the expression of HIF 1α mRNA and tumor histological differentiation ( P <0.001). Conclusion The overexpression of HIF 1α may play an important role in oncogenesis of epithelial ovarian tumor. Tissue microarray is an efficient technique of molecular biology.