干旱胁迫对西红柿根系水导的影响研究

Comparison research on water transportation of non-drought and drought-stressed tomato root systems

以 Hg Cl2 作为水通道蛋白的抑制剂 ,采用根压液流法测定干旱、未干旱胁迫下西红柿的根系水导速率和根系水分介导常数 (Lp)。结果表明 ,用 5 0 ,10 0μm ol/ L Hg Cl2 处理根系 ,未干旱胁迫株根系水导速率分别较对照下降了 4 6 .7%和 6 5 .6 7% ,用 5 0 0 ,10 0 0μmol/ L ME处理 ,可分别恢复到对照的 88.6 %和 80 .5 9% ;同样条件下干旱胁迫株根系 ,水导速率分别较对照下降了 4 0 .6 7%和 5 8.82 % ,并相应恢复到对照的 72 .0 3%和 79.4 1% ;并且干旱胁迫株根系在 0 .1～ 0 .3MPa液压时 ,根系压力与水导速率 (Jv)值呈明显的线性关系 ,暗示着干旱胁迫株根系存在控制水分运输的水通道蛋白。

A pressure flux approach was used to detect rate of water transportation of non drought and drought stressed tomato root systems,when HgCl 2 is served as aquaporins inhibitor.The results indicated that rate of water transportation of non drought roots deceased respectively by 46.7%,65.67% when treated with 50,100 μmol/L HgCl 2,and recovery to 88.6%,80.59% when treated with 500,1 000 μmol/L ME.Under same conditions,rate of water transportation of drought treated root deceased by 40.67%, 72.03% correspondently when treated with 50,100 μmol/L HgCl 2,and recovery to 72.03%,79.41% when treated with 500,1 000 μmol/L ME.And drought stressed roots of Jv have obvious linear relations between 0.1 and 0.3 Mpa .All these data and phenomena above indicated that aquaporins exists in drought stressed roots and mediated water transportation.