重组人肝细胞生长因子β链体外生物学活性的测定

Bioactivity assay of recombinant human hepatocyte growth factor-β in vitro

目的 建立一种稳定灵敏的方法用于检测重组人肝细胞生长因子β链 (rhHGF β)对原代培养成年大鼠肝细胞的增生作用。方法 利用噻唑蓝 (MTT)比色方法在多种实验条件下对rhHGF β进行生物学活性检测。结果 对于体外原代培养成年大鼠肝细胞 ,rhHGF β可刺激肝细胞的增生 ,且有剂量依赖效应。 结论 确定了原代培养肝细胞检测rhHGF

Objective To work out a stable and sensitive method (as for the cell number,culture time,etc) to detect the effect of recombinant human hepatocyte growth factor-β (rhHGF-β) on the multiplication of primary culture of hepatocytes in adult rats.Methods Bioactivity of rhHGF was assayed under various conditions using MTT.Results rhHGF-β stimulated multiplication of primary adult rat hepatocytes.Conclusion The author sifted out an optimal scheme for detecting the bioactivity of rhHGF-β in primary culture of liver cells.