草木樨状黄芪抗甲硫氨酸变异系的离体筛选及鉴定

IN VITRO SELECTION AND CHARACTERIZATION OF METHIONINE-RESISTANT VARIANT IN ASTRAGAL US MELILOTOIDES PALL.

通过组织培养体系进行了草木樨状黄芪(Astragalus melilotoides Pall.)抗甲硫氨酸变异系的筛选。无菌苗幼茎切段诱导的愈伤组织经NaN_3诱变后,经过筛选获得了抗14mmol/L甲硫氨酸的变异细胞系,并分化出大量植株。再生植株已经在大田中开花结实。经分析表明:抗性细胞系脱离选择压6个月后,放在含15mmol/L甲硫氨酸的培养基上培养25天后,其相对增长率是对照的10.2倍。抗性系再生植株游离甲硫氨酸是对照的2.12倍,并且天冬族氨基酸都有明显增加。SDS-PAGE及过氧化物酶同工酶分析表明:在蛋白质及同工酶酶谱上抗性系再生植株均出现与对照不同的差异。

A variant cell line of Astragalus melilotoides Pall, resistant to 14mmol/L methionine was selected from calluses via mutagenesis with sodium azide. The plantlets were induced from the variant calluses. The regenerated variant plants were transplanted in soil and had set seeds. After the variant calluses had been cultured for 25 days in the medium containing 15mmol/L methionine, the relative growth rate was 10. 2 folds of the control's. Free amino acids of the aspartate family in the variant cell line increased obviously in comparison with the control. Analyses of SDS-PAGE pattern and isozyme pattern of peroxidase revealed the differences between the variant plants and the control's.