兔抗人NESTIN抗血清的制备与鉴定(简报)

PREPARATION AND IDENTIFICATION OF RABBIT-ANTI-HUMAN NESTIN ANTISERUM

巢蛋白(nestin)属Ⅵ类中等纤维蛋白[1].最初在神经系统发育早期发现有该蛋白的表达[2].

Nestin belongs to the class Ⅵ intermediate filament family and it is a marker for neural progenitor cells. In this work, the 3'-terminal coding sequence(396bp) of human nestin gene was cloned into pGEX-3X plasmid and introduced into BL21 E. coli cells. The GST-nestin protein was purified with an affinity column. Anti-human nestin antiserum was raised by immunizing a rabbit with the fusion protein. The high specificity of the antibody against human nestin was confirmed by western-blot and immunocytochemistry analysis.