痢疾杆菌体内诱导基因筛选方法的建立

The establishment of screening method of in vivo induced genes from Shigella flexneri2a

分别用氯霉素乙酰转移酶基因(cat)和天冬氨酸半醛脱氢酶基因(asd)作为报告基因,用小鼠肺感染模型和HeLa细胞侵袭模型来试验筛选痢疾杆菌体内诱导基因的可行性。结果表明,以asd为报告基因,用HeLa细胞侵袭试验作为筛选模型,可成功地用于筛选痢疾杆菌的体内诱导基因。

In this study,the sreening method of in vivo-induced genes of Shigella flexneri was tested by respectively using aspartic semialdehyde dehydrogenase(asd)and chloramphenicol acetyltransferase(cat)gene as the reporter with mouse model and HeLa cell model.The results indicated that the in vivo induced genes of Shigella flexneri2a would be successfully screened with asd gene as the reporter and HeLa cell model.