核糖体展示及体外分子选择与进化

In vitro selection and evolution of functional proteins by using ribosome display

核糖体展示是20世纪90年代中期发展起来的一种简便而有效的体外分子选择与进化技术。它也是第一种完全在体外进行蛋白质或多肽分子选择与进化的方法。本文主要概述了体外核糖体展示技术的建立基础、基本原理和技术特点等,并跟踪了目前该领域的最新研究进展和发展前景。

Ribosome display technology,which developed in the middle of1990,becomes a simple and efficient tool for in vitro selection and evolution of functional molecules.Ribosome display is also the first method for screen-ing and selection of functional proteins performed completely in vitro and circumventing many drawbacks of in vivo systems.Here the author reviews the principle of ribosome display and its strategy and characteristics in applica-tions,and follows the update advances and development of the pioneering in ribosome display technology.