期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

蛋白质芯片-飞行质谱技术 被引量：3

The technique of proteinchip-surface enhanced laser desorption/ionization
下载PDF
导出
摘要 蛋白质芯片-飞行质谱技术(Proteinchip-SurfaceEnhancedLaserDesorption/Ionization,SELDI)是21世纪出现的一种新型生物芯片技术,是研究表达蛋白的有力工具。本文对该技术的原理、构成、使用方法、应用等做了简要介绍。 The technic of proteinchip-surface enhanced laser desorption/ionization that emerged in the21th century is a newly biochip technology and useful tool to research expressed proteins.In this article,we described the prin-ciple,structure,method of application and apply field of this technique.
作者 范保星 陈良安 刘又宁
机构地区 中国人民解放军总医院
出处 《生物技术通讯》 CAS 2003年第2期159-161,共3页 Letters in Biotechnology
关键词 蛋白质芯片—飞行质谱技术 SELDI 生物芯片技术 原理 构成 使用方法 proteinchip mass spectrum technique
分类号 Q51 [生物学—生物化学] Q503 [生物学—生物化学]
  • 相关文献

参考文献13

  • 1MacBeath G;Schreiber SL.Printing proteins as microarrays for high-throughput function determination[J],2000(5485).
  • 2Duddy D;Rather M.Template-imprinting nanostructured surface for protein recognition[J],1999.
  • 3Merchant M;Weinberger SR.Recent advancements in surfaceenhanced laser desorption/ionization-time of flight-mass spectrometry[J],2000(06).
  • 4Kathryn S.Finggerprinting disease with protein chip assays,1999(05).
  • 5Horninger W;Reissigl A;Rogatsch H.Prostate cancer screening in the Tyrol,Austria: experience and results,2000(10).
  • 6Wright GL;Cazares LH;Leung SM.ProteinChip surface enhanced laser desorption/ionization (SELDI) mass spectrometry:a novel protein biochip technology for detection of biomarker in complex protein mixtures,2000.
  • 7Vlahou A;Schellhammer PF;Mendrinos S.Development of a novel proteomic approach for the detection of transitional cell carcinoma of the bladder in urine[J],2001(04).
  • 8Frears ER;Stephens DJ;Walters CE.The role of cholesterol in the biosynthesis of beta-amyloid,1999(08).
  • 9Davies H;Lomas L;Austen B.Profiling of amyloid beta peptide variants using SELDI Protein Chip arrays[J],1999(06).
  • 10Austen BM;Frears ER;Davies H.The use of seldi proteinchip arrays to monitor production of Alzheimer′s betaamyloid in transfected cells[J],2000(09).

共引文献1

同被引文献35

  • 1[1]O'Farrell PH. High resolution two-dimensional electrophoresis of proteins[J]. J Biol Chem, 1975, 250(10): 4007-4021.
  • 2[2]Ong SE, Pandey A. An evaluation of the use of two-dimensional gel electrophoresis in proteomics[J]. Biomol Eng, 2001, 18(5):195-205.
  • 3[3]Gorg A, Boguth G, Kopf A et al. Sample prefractionation with Sephadex isoelectric focusing prior to narrow pH range two-dimensional gels[J]. Proteomics, 2002, 2(12): 1652-1657.
  • 4[4]Wildgruber R, Harder A, Obermaier C et al. Towards higher resolution: two-dimensional electrophoresis of Saccharomyces cerevisiae proteins using overlapping narrow immobilized pH gradients[J]. Electrophoresis, 2000, 21(13): 2610-2616.
  • 5[5]Pedersen SK, Harry JL, Sebastian L et al. Unseen proteome: mining below the tip of the iceberg to find low abundance and membrane proteins[J]. J Proteome Res, 2003, 2(3):303-311.
  • 6[6]Unlu M, Morgan ME,Minden JS. Difference gel electrophoresis: a single gel method for detecting changes in protein extracts[J]. Electrophoresis, 1997, 18(11):2071-2077.
  • 7[8]Zhou G, Li H, DeCamp D et al. 2D differential in-gel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers[J]. Mol Cell Proteomics, 2002, 1(2):117-124.
  • 8[9]Link AJ, Eng J, Schieltz DM et al. Direct analysis of protein complexes using mass spectrometry[J]. Nat Biotechnol, 1999, 17(7):676-682.
  • 9[10]Washburn MP, Wolters D, Yates JR et al. Large-scale analysis of the yeast proteome by multidimensional protein identification technology[J]. Nat Biotechno, 2001, 19(3): 242-247.
  • 10[11]Wu CC, MacCoss MJ. Shotgun proteomics: tools for the analysis of complex biological systems[J]. Curr Opin Mol Ther, 2002, 4(3): 242-250.

引证文献3

二级引证文献4

生物技术通讯
2003年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部