几种木腐菌漆酶活性的研究

Study on Laccase Activities of Wood-rot Fungi

对18种木腐菌胞外漆酶活性的测定结果表明,腐木生侧耳的漆酶活力最高,为605.0u·mL-1,其次是多毛拴菌和云芝,其漆酶活力分别为440.0u·mL-1和400.0u·mL-1,其余菌株活性低于336.7u·mL-1。腐木生侧耳漆酶作用的最适pH值为4.6,Cu2+、Mg2+、Na+、Co2+对漆酶活力有激活作用,Ag+、K+、Ca2+、Hg2+、Fe2+、Cl-和Zn2+对其漆酶活力有明显抑制作用。

In this paper, the laccase activities of 18 woodrot fungi were studied. The results showed that the maxmum activity of laccase of Pleurotus lignetilis was 605.0 u·mL-1, then the maxmum activity of laccase of Trametes trogii and Corilus versicolar were 440.0 u·mL-1 and 400.0 u·mL-1. Different pH values and inorganic ions had different effects on laccase activities of Pleurotus lignetilis. The optimum pH value of laccase of Pleurotus lignetilis was 4.6, Cu2+ ?Mg2+?Na+?Co2+ increased laccase activities whereas Ag+?K+?Ca2+?Hg2+?Fe2+ ?Cl- and Zn2+ significantly inhibited it.