期刊文献+

重症急性呼吸综合征病例尸解肺组织中新型冠状病毒部分聚合酶基因的序列测定 被引量:7

Determination of the partial polymerase gene sequence of novel coronavirus isolated from lung tissue of SARS patients
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摘要 目的 :从患者尸解肺组织中直接扩增冠状病毒部分聚合酶基因序列 ,为重症急性呼吸综合征 (非典型肺炎 )的病原确认提供依据。方法 :采用RT_PCR方法 ,从非典型肺炎病例标本中进行冠状病毒聚合酶基因的扩增与序列测定。采用ClustalX1.8软件进行系统发生树分析。结果与结论 :从非典型肺炎病例尸解肺组织、传代发病小鼠肺组织及分离物感染的细胞培养物中 ,可分别扩增出冠状病毒的DNA片段。测序结果显示其核苷酸序列与目前已知冠状病毒的同源性在 6 0 %左右 。 Objective:To amplify and determine the sequence of partial polymerase gene of novel coronavirus isolated from lung tissue of severe acute respiratory syndrome(SARS)patients in China's Mainland, and to confirm SARS pathogen.Methods:DNA fragments of the polymerase gene were obtained by reverse transcription_polymerase chain reaction (RT_PCR) and the sequence homology and phylogenetic were analysed by the Neighbour Joining method of Clustal X 1.8.Results and Conclusions: The DNA fragments with expected length could be amplified by RT_PCR from the lung tissue of SARS patients and that of infected mouse and the cell cultures. The sequence homology analyses revealed that the sequence had about 60% homology compared with other known coronaviruses, and had 99% homology with novel coronaviruses isolated in USA and Canada.
出处 《军事医学科学院院刊》 CSCD 北大核心 2003年第2期81-83,共3页 Bulletin of the Academy of Military Medical Sciences
关键词 重症急性呼吸综合征 非典型肺炎 尸体解剖 冠状病毒 聚合酶 基因序列 severe acute respiratory syndrome atypical pneumonia pathogen coronavirus RT_PCR sequence analysis
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参考文献2

  • 1Stephensen CB, CaseBolt DB, Gangopadhyay NN. Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay[J]. Virus Res,1999, 60(1): 181 - 189.
  • 2Vabret A, Mouthon F, Mourez T, et al. Direct diagnosis of human respiratory coronaviruses 229E and OC 43 by the polymerase chain reaction[Jl. J Virol Methods,2001, 97(1): 59-66.

同被引文献12

  • 1[3]Thomas G. Ksiazek, Dean Erdman, Cynthias G et al. A novel coronavirus associated with severe acute respiratory syndrome. The new Eng land Journal of medicine,2003,348(20) : 1 953
  • 2[1]Dromas GK, Cynthia G. A novel coronvirus associated with severe acute respiratory syndrome. N Engl J Med, Available at on April 10,2003
  • 3[2]Drosten C, Gunther S, Preiser W. Identification of a novel coronavirus in patient with severe acute respiratory syndrome. N Engl J Med,2003,348(15):1 967
  • 4CDC. Outbreak of severe acute respiratory syndrome-worldwide,2003[J]. Morbidity and Mortality Weekly Report, 2003, 52: 241.
  • 5WHO. Cumulative number of reported cases (SARS)[J/OL].http://www.who.int/csr/sarscountry / 2003-04-03.
  • 6Marco A M, Steven J M, Caroline R A, The genome sequence of the SARS-associated coronavirus[J/OL], http://www.scienceexpress. org/1May2003/page 1/10.1126/science. 1085953.
  • 7丁志芬,石慧颖,庞成华,常振彦,赵敏,李荆,杨抗抗,刘培生.转瓶培养非洲绿猴肾传代细胞生产纯化流行性乙型脑炎疫苗的研究[J].中华医学杂志,1998,78(4):261-262. 被引量:7
  • 8王翠娥,秦鄂德,甘永华,李豫川,吴小红,曹军田,于曼,司炳银,严格,李金风,祝庆余.非典型肺炎标本感染乳鼠和Vero E6细胞的病理学观察[J].解放军医学杂志,2003,28(5):383-384. 被引量:36
  • 9祝庆余,秦鄂德,王翠娥,于曼,司炳银,范宝昌,常国辉,彭文明,杨保安,姜涛,李豫川,邓永强,刘洪,甘永华.非典型肺炎病例标本中新型冠状病毒的分离与鉴定[J].中国生物工程杂志,2003,23(4):106-112. 被引量:100
  • 10祝庆余,秦鄂德,于曼,司炳银,杨保安,刘洪,吕富双,常国辉,彭文明,范宝昌,邓永强,韩伟国,石玉玲,李林海,张泮河,赵秋敏,曹务春.新分离的冠状病毒与严重急性呼吸综合征病原关系的研究[J].解放军医学杂志,2003,28(6):477-479. 被引量:11

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