在单一提取系统中同时测定植物ASA/DHA和GSH/GSSG

Simultaneous measurement of ASA/DHA and GSH/GSSG using a single extraction system

文章介绍了在一个单一提取系统—— 5 %磺基水杨酸中同时提取并测定了抗氧化剂 ASA/DHA和 GSH/GSSG,通过 DTNB— GR循环的动力学分析固定了 GSH/GSSG测定的最佳时间参数 ;通过与原提取和测定方法的比较 ,此法对于测定抗氧化剂有很好的准确性、重复性和可比性 ,使抗氧化剂的提取和测定变得简便快速。

This paper introduced a single antioxidant extraction system--5% sulphosalicylic acid which could extract and assay ASA/DHA and GSH/GSSG simultaneously. Through analysis of kinetic curve of DTNB-GR recycling, the suitable time parameter was selected for the measurement GSH/GSSG. This system made the extraction of antioxidant quick and easy, and enhanced the accuracy, reproducibility and comparability for the antioxidant measurement through it compared to original method of extraction and measurement.